BACKGROUND Permanent slide preparation of nematodes especially small ones is time consuming, difficult and they become scarious margins. Regarding this problem, a modified double glass mounting method was developed and compared with classic method. METHODS A total of 209 nematode samples from human and animal origin were fixed and stained with Formaldehyde Alcohol Azocarmine Lactophenol (FAAL...

Whole mount in situ hybridization is one of the most widely used techniques in developmental biology. Here, we present a high-resolution double fluorescent in situ hybridization protocol for analyzing the precise expression pattern of a single gene and for determining the overlap of the expression domains of two genes. The protocol is a modified version of the standard in situ hybridization usi...

LUCITE NOT A SUBSTITUTE FOR CANADA BALSAM WHEN MOUNTING MICROSCOPE SLIDES' LUCITE2 is a crystal-clear methyl methacrylate polymere which has a refractive index nearly the same as glass and is readily soluble in dioxan. Dioxan3 has proven useful as a dehydrating agent for the preparation of sections and objects to be mounted for observation with the microscope. Thus with the Lucite in dioxan sol...

100 random fecal samples sent to the clinical laboratory of the Aga Khan University Hospital were used. Microscopy was done by direct wet mount in which approximately 2 mg of feces (the amount that usually adheres to one end of the wooden applicator stick) was thoroughly emulsified on a glass slide in one dropofphysiologic salineandcovered with acoverslip. A similar preparation was made on anot...

1.Brugal, G. 1995. Quantitative microscopy and tumor cell proliferation. Bull. Cancer 511S517S. 2.Doyle, A., J.B. Griffiths and D.G. Newell. 1993. Cell and Tissue Culture: Laboratory Procedures. John Wiley & Sons International, Chichester, UK. 3.Gerdes, J., U. Schwab, H. Lemke and H. Stein. 1983. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cel...

The conventional K-Ar dating method was applied to the 1986 dacite flow from the new lava dome at Mount St. Helens, Washington. Porphyritic dacite which solidified on the surface of the lava dome in 1986 gives a whole rock K-Ar “age” of 0.35±0.05 million years (Ma). Mineral concentrates from the dacite which formed ins 1986 give K-Ar “ages” from 0.34±0.06 Ma (feldspar-glass concentrate) to 2.8±...

In this research, an analytical method for quantification of the thermal energy performance improvement for a building integrated photovoltaic double-skin façade has provided. The system has been suggested as a retrofit measure for an existing building in Tehran. The effect of thermal performance has been analyzed through computer-assisted developed code using engineering equation solver softwa...

The glass transition in binary mixtures of star polymers is studied by mode coupling theory and extensive molecular dynamics computer simulations. In particular, we have explored vitrification in the parameter space of size asymmetry δ and concentration F2 of the small star polymers at fixed concentration of the large ones. Depending on the choice of parameters, three different glassy states ar...