background : in this study, we used both its1 and its2 for molecular identification of fasciola species. methods : the region between 18s and 28s of ribosomal dna was used in pcr-rflp method for molecular identification of fasciola species. ninety trematodes of fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from khorasan, east azerbaijan, a...

For the better part of century, microbes have been a treasure trove for deciphering inner workings cell, from early insights into DNA replication and restriction-enzyme-mediated antiviral responses, to unravelling complexities metabolic pathways understanding gene expression its regulatory mechanisms [...]

Aims: Detection and isolation of restriction endonucleases like topoisomerase Thermophilic Bacillus subtilus strain RC1. Methods and results: Topoisomerase like enzyme activity is detected from thermophilc Bacilus sp. The isolated enzyme is partially characterized. The molecular weight of the enzyme is ~ 33 KDa and the optimum temperature for the activity is 37 0 C. The crude and gel purified e...

Cleavage of a DNA replication fork leads to fork restoration by recombination repair. In prokaryote cells carrying restriction-modification systems, fork passage reduces genome methylation by the modification enzyme and exposes the chromosome to attack by the restriction enzyme. Various observations have suggested a relationship between the fork and Type I restriction enzymes, which cleave DNA ...

dna amplification using taq dna polymerase is one of the most widely used techniques in molecular biology and biotechnology. the aim of this study was to amplify the gene of this enzyme from a thermophilic bacteria called thermus aqauticus and clone it into a vector for future use. using specific primers the cdna of taq dna polymerase was amplified and ligated into the cloning vector ptz57r usi...

Genomic DNA extraction with a high quantity and quality is a basic requirement in molecular biology. The DNA obtained was free of any contamination proteins, polysaccharide, polyphenols and colored pigments. These compounds would interfere with the genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The isolated genomic DNA was fo...

deep-seated fungal infection present with non specific symptoms and involove a large number of different organisms. dna-based technology offers for eariler detection of fungal pathogens and then earlier initiation of antifungal therapy. in this study universal primers common to almost all fungi were used to amplification of internal transcribe spacer 1 and 2 region. subsequent restriction enzym...

The restriction-modification systems use epigenetic modification to distinguish between self and nonself DNA. A modification enzyme transfers a methyl group to a base in a specific DNA sequence while its cognate restriction enzyme introduces breaks in DNA lacking this methyl group. So far, all the restriction enzymes hydrolyze phosphodiester bonds linking the monomer units of DNA. We recently r...