Background Male infertility is a multifactorial syndrome encompassing a wide variety of disorders. In more than half of infertile men, the cause of their infertility is unknown (idiopathic) and could be congenital or acquired. OGG1 is a bifunctional glycosylaseii, in that it has both AP lyase and DNA glycosylase activity and one of its functions is to excise and remove 8-oxo-dGuo from DNA. Amon...

This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymor...

DNA polymorphism within diacylglycerol transferase 2 (DGAT2) / monoacyl glycerol transferases 2 (MOGAT2), leptin and butyrophilin genes were analysed using PCR-SSCP in Murrah buffalo. The single strand conformation polymorphism (SSCP) analysis of amplified gene fragment in exon 5 of MOGAT2, exon 3 of leptin and intron 1 of butyrophilin gene revealed different patterns. A, B and C showed the fol...

Background: Single nucleotide polymorphisms in 8-oxoguanine DNA glycosylase-1 (OGG1) gene modulates DNA repair capacity and functions as one of the first lines of protective mechanisms against 8-hydroxy-2’-deoxyguanosine (8-OHdG) mutagenicity. OGG1-Cys326 gene polymorphism may decrease DNA repair function, causing oxidative stress due to higher oxidative DNA damage. The main purpose of this stu...

An attempt has been made to analyze the distribution of the beta-lactoglobulin genotype in swamp buffaloes and Murrah buffaloes utilizing polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Blood samples were taken from 50 swamp buffaloes and 50 Murrah buffaloes. The DNA was extracted by the phenol-chloroform method. The PCR-RFLP was performed using beta-lactoglobulin ...

Cytokines are secreted or membrane-bound proteins that act as mediators of intercellular signaling to regulate homeostasis the immune system. They produced by cells innate and adaptive immunity in response microbes tumor antigens. Although there several studies showing IL2-330 gene polymorphism is associated with many types cancer, far we know, a few study investigating association between lung...

Background: One of the most important phenotypic modifying factors for thalassemia is the presence of Xmn1 polymorphism. This retrospective study was performed to investigate the overall prevalence of Xmn1 polymorphism among Iranian β-thalassemia patients with homozygote IVSII-1mutation and to assess the relationship between Xmn1 polymorphism with patients’ hemoglobin levels and the response to...