نتایج جستجو برای: dna extraction
تعداد نتایج: 663961 فیلتر نتایج به سال:
Nosocomial infection caused by methicillin-resistant staphylococci poses a serious problem in many countries. The aim of this study was to rapidly and reliably detect methicillin-resistant-staphylococci in order to suggest appropriate therapy. The presence or absence of the methicillin-resistance gene in 115 clinical isolates of Staphylococcus aureus and 50 isolates of Coagulase Negative Staphy...
background: entamoeba moshkovskii and e. dispar are impossible to differentiate microscopically from the pathogenic species e. histolytica . multiplex polymerase chain reaction (multiplex pcr) is a widespread molecular biology technique for amplification of multiple targets in a single pcr experiment. methods: for detection and differentiation of the three-microscopy indistinguishable entamoeba...
detection of genetically modified organisms (gmos) in food is an important issue for all the subjects involved in food control and customer’s right. due to the increasing number of gmos imported to iran during the past few years, it has become necessary to screen the products in order to determine the identity of the consumed daily foodstuffs. in this study, following the extraction of genomic ...
DNA extraction from plant tissues often causes most problems. For example, unsuccessful removal secondary metabolites during extraction, such as phenolic compounds in aromatic and medicinal plants, cause to some mistakes in result of molecular experiments by using of the extracted DNA. Achillea wilhelmsii is a medicinal plant belong to Asteraceae family and native to Iran, there is little infor...
Abstract Background and objectives: Genomic DNA extraction of bacterial cells is of processes performed normally in most biological laboratories therefore, various methods have been offered, manually and kit, which may be time consuming and costly. In this paper, genomic DNA extraction of Staphylococcus aureus was investigated using some laundry detergent brands available in Iran to achieve ...
Background and purpose: Giardia lamblia (G.lamblia) is one of flagellated protozoan pathogens. Giardiasis is the most common human parasite infection in the world, causing a lengthy course of diarrhea. The aim of the present study was to compare five different methods of DNA extraction from G.Lamblia cyst and determining the most efficient DNA extraction method. Materials and methods: This stu...
abstract genomic dna extraction with high quantity and quality is the basic necessities of molecular genetic studies. the aim of this study is the applicability of different dna extraction methods as the easiest and fastest methods for and their efficiency with different tissue qualities (foot, siphon, harpoon) from marine cone snail conus coronatus, mollusca with phenol-chloroform, ammonium ac...
High-quality DNA extraction plays an important role to make sharp bands in the gel electrophoresis and also produces clean chromatograms. Usually, DNA extract is delivered using the modified CTAB method but this method cannot obtain high-quality DNA for molecular analysis from old dried leaves of Bellevalia due to having different chemical compounds which inhibit to obtain a clear DNA extractio...
introduction: dna extraction methods are very important for genetics research and diagnostic tests. in these methods are also important for detecting genetic diseases or in cancers for investigating genetic changes during cancer progression or treatment. therefore, selection of the best method for dna extraction from different samples such as bone marrow (bm) or peripheral blood (pb) and their ...
background: fetal dna in maternal plasma and serum has been shown to be a useful material for prenatal fetal sex determination during early gestational ages. non-invasive prenatal diagnosis is now possible at 8th week of pregnancy, by maternal blood sample testing. objective: the purpose of this study was to evaluate two dna extraction methods from mother plasma and its routine clinical applica...
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