نتایج جستجو برای: dna cleavage analysis

تعداد نتایج: 3245516  

Journal: :international journal of fertility and sterility 0
seda yılmaz asuman demiroglu zergeroğlu elif yılmaz kenan sofuoglu nuri delikara pelin kutlu

background: sperm dna fragmentation is known as an important cause of male infertility. the influence of sperm dna damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. in this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (icsi) outcome according to sperm dna fragme...

Journal: :Proceedings of the National Academy of Sciences 1978

Journal: :International Journal of Molecular Sciences 2021

Journal: :Chemical Communications 2021

We demonstrated the photo-controlled sequence-selective dsDNA cleavage using a DNA origami structure with Cas9 nuclease.

2007
Petra Volná Jordan Jarjour Sarah Baxter Steve R. Roffler Raymond J. Monnat Barry L. Stoddard Andrew M. Scharenberg

LAGLIDADG homing endonucleases (LHEs) cleave 18-24 bp DNA sequences and are promising enzymes for applications requiring sequence-specific DNA cleavage amongst genome-sized DNA backgrounds. Here, we report a method for cell surface display of LHEs, which facilitates analysis of their DNA binding and cleavage properties by flow cytometry. Cells expressing surface LHEs can be stained with fluores...

Journal: :Nucleic acids research 1985
M Sander T S Hsieh

In order to study the sequence specificity of double-strand DNA cleavage by Drosophila topoisomerase II, we have mapped and sequenced 16 strong and 47 weak cleavage sites in the recombinant plasmid p pi 25.1. Analysis of the nucleotide and dinucleotide frequencies in the region near the site of phosphodiester bond breakage revealed a nonrandom distribution. The nucleotide frequencies observed w...

Journal: :Journal of the American Chemical Society 2006
Jens Brunner Jacqueline K Barton

The DNA photocleavage reaction of mismatch-selective Rh complexes has been analyzed by MALDI-TOF mass spectrometry as well as gel electrophoresis analysis of radioactively tagged oligonucleotides. Analogous results are obtained with these two techniques showing site-specific cleavage neighboring the mismatch to yield primarily 5'- and 3'-phosphate termini. Additional intermediates and products ...

2017
Brendan J. Hilbert Janelle A. Hayes Nicholas P. Stone Rui-Gang Xu Brian A. Kelch

Many viruses use a powerful terminase motor to pump their genome inside an empty procapsid shell during virus maturation. The large terminase (TerL) protein contains both enzymatic activities necessary for packaging in such viruses: the adenosine triphosphatase (ATPase) that powers DNA translocation and an endonuclease that cleaves the concatemeric genome at both initiation and completion of ge...

2013
Elisa Arnoldi Xiao-Su Pan L Mark Fisher

Antibacterial fluoroquinolones trap a cleavage complex of gyrase and topoisomerase (topo) IV inducing site-specific DNA breakage within a bent DNA gate engaged in DNA transport. Despite its importance for drug action and in revealing potential sites of topoisomerase catalysis, the mechanism of DNA selectivity is poorly understood. To explore its functional basis, we generated mutant versions of...

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