We report on the size, shape, structure, and interactions of lysozyme in the ternary system lysozyme/DMSO/water at low protein concentrations. Three structural regimes have been identified, which we term the "folded" (0 < φ(DMSO) < 0.7), "unfolded" (0.7 ≤ φ(DMSO) < 0.9), and "partially collapsed" (0.9 ≤ φ(DMSO) < 1.0) regime. Lysozyme resides in a folded conformation with an average radius of g...

This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at differen...

Niemann-Pick type C (NPC) fibroblasts present a large concentration of cholesterol in their cytoplasm due to a still unidentified deficiency in cholesterol metabolism. The influence of dimethylsulfoxide (DMSO) on the amount of intracellular cholesterol was measured in 8 cultures of normal fibroblasts and in 7 fibroblast cultures from NPC patients. DMSO was added to the fibroblast cultures at th...

Surface vibrational sum frequency generation (VSFG) spectroscopy complemented with surface tension measurements has been utilized to probe the air/ dimethyl sulfoxide (DMSO) interface as a function of DMSO concentration in water. For the neat DMSO surface, the DMSO methyl groups extend away from the liquid phase and VSFG polarization studies show that the methyl transition dipole moments of pur...

The interactions between phospholipid monolayers and dimethylsulfoxide (DMSO) molecules were investigated by vibrational sum frequency generation (VSFG) spectroscopy in a Langmuir trough system. Both the head and the tail groups of dipalmitoylphosphatidylcholine (DPPC) as well as DMSO were probed to provide a comprehensive understanding of the interactions between DPPC and DMSO molecules. A con...

Regulation of the expression of dimethylsulfoxide (DMSO) reductase was investigated in the purple phototrophic bacterium Rhodobacter capsulatus. Under phototrophic, anaerobic conditions with malate as carbon source, DMSO caused an approximately 150-fold induction of DMSO reductase activity. The response regulator DorR was required for DMSO-dependent induction and also appeared to slightly repre...

The combinational treatment between the selective antimetastatic agent, sodium-trans-rutheniumtetrachloridedimethylsulfoxideimidazole, Na[trans-RuCl(4)(DMSO)Im], and the cytotoxic drug 5-fluorouracil (5-FU) on primary tumor growth and on the survival time of experimental tumors results in an effect significantly greater than that of each single agent used alone either with the solid metastasizi...

Dimethyl sulfoxide (DMSO) has anti-inflammatory and analgesic properties and is the only intravesical agent approved by the FDA for the treatment of interstitial cystitis. While it is known that DMSO has numerous biological effects on cell differentiation and alteration of cell-surface carbohydrate structures, the anti-inflammatory mechanism of DMSO has been not clear yet. Therefore, further in...

The aim of this study was to investigate the effect of different cryoprotectants like glycerol and DMSO individually and in combination of both on morphology of sheep oocyte using vitrification. The vitrification of sheep oocyte was performed using 40% glycerol and 40% DMSO individually and in combination of 20% glycerol + 20% DMSO. It was found that combination of cryoprotectants proved more e...