We find that moderate cationic selectivity of the general bacterial porin OmpF in sodium and potassium chloride solutions is inversed to anionic selectivity in concentrated solutions of barium, calcium, nickel, and magnesium chlorides. To understand the origin of this phenomenon, we consider several factors, which include the binding of divalent cations, electrostatic and steric exclusion of di...

The open reading frame SCO4226 of Streptomyces coelicolor A3(2) encodes an 82-residue hypothetical protein. Biochemical assays revealed that each SCO4226 dimer binds four nickel ions. To decipher the molecular function, we solved the crystal structures of SCO4226 in both apo- and nickel-bound (Ni-SCO4226) forms at 1.30 and 2.04 Å resolution, respectively. Each subunit of SCO4226 dimer adopts a ...

Recombinant His-tagged proteins expressed in Escherichia coli and purified by immobilized metal affinity chromatography (IMAC) are commonly coeluted with native E. coli proteins, especially if the recombinant protein is expressed at a low level. The E. coli contaminants display high affinity to divalent nickel or cobalt ions, mainly due to the presence of clustered histidine residues or biologi...

Flax shive constitutes about 70% of the flax stem and has limited use. Because shive is a lignocellulosic by-product, it can potentially be pyrolyzed and activated to produce an activated carbon. The objective of this study was to create an activated carbon from flax shive by chemical activation in order to achieve significant binding of selected divalent cations (cadmium, calcium, copper, magn...

The modulation of solubilized type 1 and type 2 benzodiazepine receptors from cow brain by gamma-aminobutyric acid (GABA), divalent cations, and anions has been evaluated. GABA stimulates [3H] flunitrazepam binding of both receptor subtypes, whereas divalent cations and anions selectively stimulate solubilized type 2 receptors. Of numerous anions examined, only chloride, bromide, and iodide enh...

Hydrogenases are generally classified as iron-sulfur-containing proteins with four to twelve iron atoms in different cluster arrangements. Through physiological, chemical and spectroscopic studies,nickel has also been found to be a constitutive metal of several hydrogenases and shown to be involved in a redox linked process. The [NiFe] hydropnnses are now the most intensivelly studied nickel en...

The aim of the present study was to synthesize and characterize divalent metal ions Nickel, Copper Zinc complexes 5-chloro 2-furaldehyde thiosemicarbazone. Firstly ligand prepared from reaction between 5-Chloro-2-furaldehyde thiosemicarbazide, second step involved synthesis Ni(II), Cu(II) Zn(II) compounds (ligand or complex) characterized using different analytical techniques such as elemental ...

ron phosphate coatings have found a wide range of use in the metalI finishing industry as a convenient method for achieving reasonably high levels of corrosion resistance. Flexibility of processing parameters and ease of use are probably the biggest reasons that iron phosphating enjoys its continued popularity. The corrosion resistance of iron phosphate coatings can be dramatically improved by ...