Phenotypic assays using human primary cells are highly valuable tools for target discovery and validation in drug discovery. Expression knockdown (KD) of such targets in these assays allows the investigation of their role in models of disease processes. Therefore, efficient and fast modes of protein KD in phenotypic assays are required. The CRISPR/Cas9 system has been shown to be a versatile an...

Affinity chromatography and immunolocalization techniques were used to investigate the mechanism(s) by which endothelial cells interact with the basement membrane component laminin. Bovine aortic endothelial cells (BAEC) membranes were solubilized and incubated with a laminin-Sepharose affinity column. SDS-PAGE analysis of the eluted proteins identified a 69-kD band as the major binding protein...

Construction of effective acceleration structure is an essential and challenging problem in ray tracing. The surface area heuristic (SAH), regarded as the standard cost function for construction, is based on the assumption that rays are uniformly distributed. This simplification ignoring actual ray distribution results in a reduction both on construction and rendering performance. Unlike previo...

Separate phases of metal partitioning behaviour in freshwater lakes that receive varying degrees of atmospheric contamination and have low concentrations of suspended solids were investigated to determine the applicability of the distribution coefficient, KD. Concentrations of Pb, Ni, Co, Cu, Cd, Cr, Hg and Mn were determined using a combination of filtration methods, bulk sample collection and...

co-channel interference is a major factor in limiting the capacity and link quality in cellular communications. as the co-channel interference is modeled by lognormal distribution, sum of the co-channel interferences of neighboring cells is represented by the sum of lognormal random variables (rvs) which has no closed-form expression. assuming independent, identically distributed (iid) rvs, the...

Fibronectin binding sites on cultured human fibroblasts were localized by high voltage electron microscopy using either 5- or 18-nm colloidal gold beads (Au5 or Au18) bound to intact fibronectin, the 70-kD amino-terminal fragment of fibronectin that blocks incorporation of exogenous fibronectin into extracellular matrix, or 160-180-kD fragments of fibronectin with cell adhesion and heparin-bind...

introduction: international study on asthma was conducted to study the prevalence of asthma symptoms among 13-14 year old children using written and video questionnaires during the early 90's. the aim of the present study (isaac) was to evaluate the agreement between the two questionnaires which were self-completed by the children. methods: this study, which was a part of the third phase o...