The purification of oligonucleotides by ion-exchange displacement chromatography is demonstrated on the gram-scale. Using a 50 mmD x 100 mmL (203 ml) column operated in the displacement mode, 1.2 g of a 24mer phosphorothioate oligonucleotide was purified. Product yield for this separation was 70% (780 mg) at a purity of 96.4% and the mass balance recovery of all oligonucleotide was 97.5%. The d...

The antibody fraction (less than 6ml) was added to immobilized enzyme (2ml of packed gel). The antibodies were recycled for 2h and the unattached antibodies washed from the gel for 1 h. Specific antibodies were eluted by washing with citric acid (0.1 M, pH2.2). Experiments marked with an asterisk were performed simultaneously. The molecular weights of immunoglobulin G and creatine kinase were t...

Chromatographic separations of amino acids on ion exchange resins may be performed on a preparative scale either by elution (24) or by displacement (5-12). Some of the exceptional resolving power demonstrated in analytical separations by elution techniques (13, 14) disappears when larger columns with relatively higher loads are used (2). Displacement chromatography allows large quantities of so...

Chromatographic separations of amino acids on ion exchange resins may be performed on a preparative scale either by elution (24) or by displacement (5-12). Some of the exceptional resolving power demonstrated in analytical separations by elution techniques (13, 14) disappears when larger columns with relatively higher loads are used (2). Displacement chromatography allows large quantities of so...