نتایج جستجو برای: direct labelling
تعداد نتایج: 437032 فیلتر نتایج به سال:
BACKGROUND beta-III-neurotubulin is a characteristic degradation protein of cellular cytoskeleton of nervous tissues, which originates with disorders that lead to the loss of peripheral neurons or neurons of the central nervous system. For the diagnostics of neuropathic and neurodegenerative processes, beta-III-neurotubulin is therefore the goal structure, and radioactive labelled antibody TU-2...
We demonstrate an approach to optical DNA mapping, which enables near single-molecule characterization of whole bacteriophage genomes. Our approach uses a DNA methyltransferase enzyme to target labelling to specific sites and copper-catalysed azide-alkyne cycloaddition to couple a fluorophore to the DNA. We achieve a labelling efficiency of ∼70% with an average labelling density approaching one...
OBJECTIVE To compare direct and indirect bead-based T-cell isolation followed by magnetic bead-based DNA isolation. METHODS T-cells were isolated by direct or indirect selection with magnetic bead coated antbiodies followed by magnetic bead-based automated DNA isolation in 10 healthy subjects. Purity of T-cells, purity of DNA (by A260/A280 ratio measurement) and DNA concentration were assesse...
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We develop an unsupervised semantic role labelling system that relies on the direct application of information in a predicate lexicon combined with a simple probability model. We demonstrate the usefulness of predicate lexicons for role labelling, as well as the feasibility of modifying an existing role-labelled corpus for evaluating a different set of semantic roles. We achieve a substantial i...
The ligand-dependent incorporation of a reporter molecule (e.g., fluorescence dye or biotin) onto a endogenous target protein has emerged as an important strategy for elucidating protein function using various affinity-based labelling reagents consisting of reporter, ligand and reactive units. Conventional labelling reagents generally use a weakly activated reactive unit, which can result in th...
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