By ROBERT E. ANDERSON AND Ro L. WALFORD T HE PRESENT study forms part of a continuing effort to develop quantitative methods for detecting antigen-antibody reactions involving nucleated cells as antigen. Here we shall present information regarding the preparation of purified, 1131-labelled anti-human-globulin antibody (AHG antibody) by means of adsorption and elution from normal human globulin,...

Erythrocytes from mink chronically infected with Aleutian disease virus (ADV) gave positive antiglobulin reactions with rabbit anti-mink immunoglobulin (Ig)G, anti-mink C3, and anti-mink serum, but did not react with anti-mink IgM. The strongest reaction was observed with anti-mink C3. Immunoelectrophoresis demonstrated that serum from rabbits injected with erythrocytes from ADV-infected mink g...

polyclonal anti-thymocyte globulin (atg) is used as an immunosuppressive agent in the treatment of aplastic anemia (aa). serum sickness is a recognized side effect of atg. we observed abnormal skin manifestation in patient with aplastic anemia who had been treated with atg. we conclude that abnormal immune function caused by aplastic anemia and atg and corticosteroids may aggravate the signs of...

Even though clinical features in autoimmune haemolytic anaemia vary according to the type of AIHA, anaemic syndrome stays common for most cases. A positive Coombs test or direct anti-globulin developed 1945 by Coombs, Mourant and Race, is deciding factor AIHA diagnosis. Since immunologic mechanisms causing erythrocyte destruction between AIHAs, treatment also different. Empirical approach with ...

Tetanus toxoid elicits protective antibodies against tetanus toxin in humans and animals. It has been reported that antitoxin from immunized humans contains no anti-light chain antibodies, based on immunodiffusion and quantitative precipitin analyses. We confirmed the absence of precipitating anti-light chain antibodies in tetanus immune globulin. However, the presence of antibodies against the...

OBJECTIVE To evaluate signs of haemolysis in babies of Rh-D negative mothers who underwent prophylaxis with anti-D immunoglobulin during pregnancy. DESIGN The following were evaluated in all babies of Rh-D negative mothers born within a three month period in our department: haemoglobin level, packed cell volume, mean corpuscular volume, reticulocytes, bilirubin level, and direct Coombs' test ...

methods this prospective observational study was conducted in a tertiary level neonatal intensive care unit, princess esra hospital, deccan college of medical sciences, hyderabad, telangana, india. consecutive intramural and extramural neonates admitted to neonatal intensive care unit with the rh-negative mother’s blood group and hyperbilirubinemia were enrolled. neonates born to rh+ve mothers ...

A simple anti-gamma-globulin consumption test for use in suspected Hashimoto's disease is described. Results in cases with positive and negative tanned red-cell tests are described.