Phloem exudate collected from hybrid poplar (Populus trichocarpa x Populus deltoides) was estimated to have more than 100 proteins, of which 48 were identified using LC-MS/MS. Comparative two-dimensional gel electrophoresis demonstrated that two phloem exudate proteins were significantly (P<0.05) upregulated 24 h after leaf wounding. These were identified as pop3/SP1 and a thaumatin-like protei...

Soluble cytoplasmic extracts of Candida albicans groups A and B were prepared and compared by quantitative immunoelectrophoresis experiments performed with a commercial anti-C. albicans group A immune serum. Although crossed immunoelectrophoresis, tandem crossed immunoelectrophoresis, and line immunoelectrophoresis revealed many cross-reactions between the two groups, some components seemed to ...

Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.

The amount of 140 individual proteins of E. coli B/r was measured during balanced growth in five different media. The abundance of each protein was determined from its absolute amount in 14C-glucose-minimal medium and a measurement of its relative amount at each growth rate using a double labeling technique. Separation of the proteins was carried out by two-dimensional gel electrophoresis. This...

A new method for spot matching in two-dimensional gel electrophoresis images using a cumulative frequency matrix is proposed. The method improves on the weak points of the previous method called 'spot matching by topological patterns of neighbour spots'. It accumulates the frequencies of neighbour spot pairs produced through the entire matching process and determines spot pairs one by one in or...

exact solution for steady two-dimensional flow of an incompressible magma is obtained. themagmatic flow is studied by considering the magma as a second grade fluid. the governing partialdifferential equations are transformed to ordinary differential equations by symmetry transformations. resultsare discussed through graphs to understand the rheology of the flowing magma

A subset W of the vertices of a graph G is a resolving set for G when for each pair of distinct vertices u,v in V (G) there exists w in W such that d(u,w)≠d(v,w). The cardinality of a minimum resolving set for G is the metric dimension of G. This concept has applications in many diverse areas including network discovery, robot navigation, image processing, combinatorial search and optimization....

In this paper, a two-dimensional multi-wavelet is constructed in terms of Chebyshev polynomials. The constructed multi-wavelet is an orthonormal basis for space. By discretizing two-dimensional Fredholm integral equation reduce to a algebraic system. The obtained system is solved by the Galerkin method in the subspace of by using two-dimensional multi-wavelet bases. Because the bases of subs...