The acyl group of acyl dihydroxyacetone phosphate was shown to be enzymatically transferred in guinea pig liver mitochondria to various acceptors such as lysolecithin, lysophosphatidyl ethanolamine and sn-glycerol3-phosphate to form lecithin, phosphatidyl ethanolamine and phosphatidate, respectively. Coenzyme A and Mg*, but not ATP, were required for this reaction. A rapid exchange of acyl grou...

Pahnitoyl dihydroxyacetone phosphate and [1-14C]hexadecanol were found to serve as precursors of 0-alkyl lipids in a microsomal system from Ehrlich ascites tumor. Both acyldihydroxyacetone phosphate and dihydroxyacetone phosphate were tested. The system required no CoA when acyldihydroxyacetone phosphate was used as the glycerol source, and significantly lower concentrations of acyldihydroxyace...

The activity of acyl-CoA: dihydroxyacetone phosphate acyltransferase in rat liver was studied by measuring the formation of labeled lipid from dihydroxyacetone [W]phosphate and either palmitoyl-CoA or a mixture of palmitate, CoA, and ATP. Bovine serum albumin stimulated the activity of the enzyme several-fold. In the presence of albumin the acyltransferase activity in mitochondria was much high...

In the first paper (1) it was shown that in normal persons dihydroxyacetone given by mouth in 25 or 50 gram doses caused a more rapid and a greater carbohydrate combustion than the same dose of glucose. The elevation of the total metabolism was more marked after the dihydroxyacetone and there was little if any increment increase in the blood sugar. In this report similar studies have been appli...

Dihydroxyacetone-phosphate:acyl coenzyme A acyltransferase (EC 2.3.1.42) was solubilized and partially purified from guinea pig liver crude peroxisomal fraction. The peroxisomal membrane was isolated after osmotic shock treatment and the bound dihydroxyacetone-phosphate acyltransferase was solubilized by treatment with a mixture of KCl-sodium cholate. The solubilized enzyme was partially purifi...

Acinetobacter sp. strain JC1 DSM 3803, a carboxydobacterium, grown on methanol was found to show dihydroxyacetone synthase, dihydroxyacetone kinase, and ribulose 1,5-bisphosphate carboxylase, but no hydroxypyruvate reductase and very low hexulose 6-phosphate synthase, activities. The dihydroxyacetone synthase was found to be expressed earlier than the ribulose 1,5-bisphosphate carboxylase. The ...

An increasing number of investigators (2-5) have postulated the occurrence of an exchange reaction catalyzed by transaldolase between glyceraldehyde S-phosphate and fructose 6phosphate to explain the unequal labeling of the 6 carbons of hexoses which is observed in tracer experiments. Transaldolase accepts the dihydroxyacetone moiety from sedoheptulose 7-phosphate or fructose B-phosphate to for...

The rate of oxidation by ferricyanide of the aldolase-dihydroxyacetone phosphate complex was measured under different conditions. The following conclusions are drawn. 1. In the cleavage of fructose diphosphate, catalysed by native aldolase, the steady-state concentration of the enzyme-dihydroxyacetone phosphate carbanion intermediate represents less than 6% of the total enzyme-substrate interme...