Euphorbia macrostegia or Persian wood spurge is one of the seventeen endemic plants of this genus in Iran. Three triterpenoids: 24-methylenecycloartan-3β-ol (1), butyrospermol (2) and cycloartenol (3) and three diglycerides: 1,2-di-O-α-linolenoyl-sn-glycerol (4), 1-O-linoleoyl-3-O-palmitoyl-sn-glycerol (5) and 1-O-α-linolenoyl-2-O-palmitoyl-sn-glycerol (6) were isolated from a hexane layer of m...

Phosphatidylethanolamine or phosphatidylcholine may be synthesized in mammalian tissues from diglycerides and cytidine diphosphate ethanolamine or cytidine diphosphate choline, respectively (1). Hydrolysis of the naturally occurring phosphatides has indicated that often the fatty acids at the @ position are unsaturated whereas those at (Y’ are more often saturated (2-4). Although it is possible...

The relationship between intestinal bile acids and the micellar solubilization of lipids was studied by determining micellar bile acids and distribution of cholesterol and 0-sitosterol, their esters, and of hydrolysis products of triglycerides (labeled with H-tristearate), free fatty acids, monoglycerides and diglycerides between the micellar and oil phases of the intestinal contents for 1% h a...

Pure preparations of phosphatidylcholine were isolated from spinach leaf chloroplasts, spinach leaf microsomes, and cauliflower inflorescence. The isolated phosphatidylcholine was treated with snake venom phospholipase A, and the fatty acid distribution and composition of the fatty acid methyl esters prepared from the lysophosphatidylcholine and the freed fatty acid were determined by gas-liqui...

S U M M A R Y The method of digestion with pancreatic lipase for determining the position of fatty acids in triglycerides has been modified to increase its accurary and ease of applicability. Moreover, by acylation of partial glycerides with a “marker” fatty acid, the method can be used for determining the structure of monoand diglycerides. Evidence is presented which demonstrates that acylatio...

Human pancreatic lipase assays are usually performed in the presence of either emulsified triglycerides or diglycerides within the limits of their solubility. Two reactions are catalyzed in the presence of triglycerides: hydrolysis of triglycerides to diglycerides, and diglycerides to monoglycerides. The contribution of each reaction to the final result was determined after extensive kinetic st...

The stereochemical course of hydrolysis of radioactive triolein was determined using lipoprotein lipase of cow’s milk and rat postheparin plasma. [3H]Glycerol trioleate or glycerol [lJ4C]trioleate was emulsified with total egg yolk lipids to provide 0.2 to 2.5 PCi of 3H or 0.03 PCi of 14C per PM substrate. The hydrolysis products were isolated at 1 to 45 min and free fatty acids and the various...

The chemical screening carried out on Schizochytrium sp. biomass led the identification of 24 types of organic compounds belonging to n-alkanes, 1-alkenes, 1-alkanols, free fatty acids, methyl and ethyl esters of saturated and unsaturated fatty acids, saturated tri- and diglycerides, unsaturated monoglycerides, wax esters, sterols, triterpenes, and mono- and sesquiterpenes. Moreover, a sample c...

A pathway for steryl ester biosynthesis in acetone powder preparations from spinach (Spinacia oleracea L.) leaves has been elucidated; free sterol and 1,2-diglyceride were the substrates. Although animals synthesize cholesteryl esters by three distinct biosynthetic pathways, none of these pathways utilizes 1,2-diglyceride as an acyl donor. Phosphatidylcholine, phosphatidic acid, triglyceride, 1...