We describe a modified colorimetric method for determining procainamide in plasma by use of a diazotization and coupling reaction with N-(1-naphthyl)-ethylenediamine dihydrochloride (Marshall's reagent). The sensitivity of the assay is increased twofold over the originally reported colorimetric method, and the lower limit of detection for the assay of a 1.0-ml plasma sample has been decreased t...

BACKGROUND The Cinnoline moiety and Sulphonamide moiety both have good antimicrobial properties. METHODS In the present study both the moieties were condensed to synthesize substituted cinnoline sulphonamide derivatives using intramolecular cyclization followed by diazotization in order to get synergistic activity. RESULTS These derivatives particularly halogen substituted cinnoline derivat...

A series of new azo amino uracil dyes were synthesized by diazotization of nine aromatic amines using sodium nitrite and hydrochloric acid and subsequent coupling to 6-aminopyrimidine-2, 4(1H, 3H) – dione. The effects of solvents, substituents, acid and base on the visible absorption maxima of the dyes were also discussed.

A versatile multistep continuous flow setup is reported for the four-step conversion of anilines into pyrazole products. The synthesis machine incorporates the use of amine-redox chemistry through diazotization and a metal-free vitamin C mediated reduction. The machine can be used for the synthesis of an array of analogues or the scale up of an individual target.

Mild, non-acidic cordons used in the n~-~u~us ~~~~ of a 2-~o~n~~~e nu&uside with t-butyi nitrite gave an unexpected ~~~~~~-2~e nuckoside which was not formed when primary a&y1 nitrites were used. The isolated compounds were evaluated for activity against herpesviruses and for cytotoxicity against human foreskin fibroblast cells. Benzimidazoles have demonstrated widespread biological activity ra...

A rapid and sensitive spectrophotometric method is proposed for determination of metronidazole and secnidazole. The method depends on the reduction of metronidazole and secnidazole molecule with zinc dust and hydrochloric acid flowed by diazotization and coupling with 8-quinolinol to give red colored chromogens easily measured spectrophotometrically which has lambda(max) = 500 nm. The experimen...

2-(5'-Phenylpentyl)fumaric acid was shown to be a competitive inhibitor (Ki 0.5 mM) of pig heart fumarase. After nitration of the aromatic ring, reduction to the amine and diazotization, the acid was attached via azo linkages to a Sepharose 4B-tyramine matrix. The resulting adsorbent was used for the affinity chromatography of crude fumarase, purifications of approx. 20-fold being obtained by s...