During June 1997-June 1999 rotavirus infection was screened in infants aged up to 2 years and hospitalised with acute diarrhoea in São Luís, Northeastern Brazil. Altogether, 128 stool samples were collected from diarrhoeic patients and additional 122 faecal specimens from age and temporal matched inpatients without diarrhoea were obtained; rotavirus positivity rates for these groups were 32.0% ...

To document the association of pathogenic Escherichia coli with diarrhea in Romanian children, 250 E. coli fecal isolates from children under 5 years of age were PCR-screened for well-recognized virulence determinants, as well as for their phylogenetic background. The putative diarrheagenic E. coli (DEC) were investigated for susceptibility to various antibiotics. Overall, 61 E. coli isolates w...

Ten out of 18 babies at risk developed enteritis in an outbreak in a special-care baby unit. Salmonella, Shigella, and Escherichia coli belonging to the traditional infantile enteropathogenic serogroups were not found in faeces from the babies and the staff, and no virus particles were found by electron microscopy. Detailed serotyping of E. coli showed that five of the 10 babies with diarrhoea ...

In a one-year prospective study carried out to define the role of rotavirus and Escherichia coli in local childhood diarrhea, we determined the prevalence of both agents in 54 diarrheic children attending a health center in Botucatu. Diarrheogenic E. coli (DEC) strains were characterized by O:H serotyping, a search for virulence genetic markers, and assays of adherence to HEp-2 cells. Except fo...

This case-control study detected and characterized Shigella and diarrheagenic Escherichia coli (DEC) types among Vietnamese children less than 5 years old. In 249 children with diarrhea and 124 controls, Shigella spp. was an important cause of diarrhea (P < 0.05). We used multiplex PCR and DNA probes to detect enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAggEC), enteropathogenic E...

E.coli O157 is one of the important causes of bloody diarrhea which can be self-limiting or may complicate into Haemolytic Ureaemic Syndrome (HUS). We are reporting a case of Enterohaemorrhagic Escherichia coli (E.coli O157) in a thirty year old male who presented to the surgical OPD of Tribhuvan University Teaching Hospital (TUTH), Kathmandu, Nepal with the complaint of bloody diarrhea and abd...

Background: Salmonella is an important foodborne pathogen that causes diarrhea in humans worldwide. Objectives: This study aimed to determine the serotype distribution, antibiotic-resistant genes, and pathogenicity island (SPI) genes of clinical isolates Weifang. Methods: A total 111 strains were collected from Weifang People’s Hospital between 2018 2020 subjected serotyping using Kauffmann-Whi...

introduction: awareness of the species of shigella has particular importance in the way of dealing with an outbreak, controlling it, and treating patients. the purpose of this study was to use the multiplex pcr method and comparison with the culture method to detect shigella species, as well as analysing the antibiotic resistance pattern in its different species. methods: simultaneously, the fe...

Stool samples were examined from 30 preterm neonates admitted to a nursery ward; 16 neonates had diarrhea, 12 constituted an age-matched control group without diarrhea, and 2 had an unknown history regarding diarrhea. Variable numbers of enteropathogenic Escherichia coli serotype O111:HNT strains possessing the gene coding for the enteroadherence factor (EAF) were found in stool samples from 13...

BACKGROUND Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a well-recognized cause of bloody diarrhea and hemolytic-uremic syndrome (HUS). Non-O157 STEC contribute to this burden of illness but have been underrecognized as a result of diagnostic limitations and inadequate surveillance. METHODS Between 1983 and 2002, 43 state public health laboratories submitted 940 human non-O157 STE...