BACKGROUND AND AIMS The effects of dextran sulphate sodium (DSS) on mouse intestinal epithelial cells and intraepithelial lymphocytes were analysed to investigate the mechanism by which DSS induces colitis and tumours in mice. Cytotoxicity of DSS towards intestinal epithelial cells and intestinal intraepithelial lymphocyte hybridomas or fresh intestinal intraepithelial lymphocytes seems to have...

Crohn’s disease is an autoimmune inflammatory bowel characterized with the chronic relapsing-remitting inflammation, mainly in terminal parts of small intestine and colon, as a result genetic predisposition or environmental factors influence. It associated impaired bacterial clearance changes immunological parameters what resulted disruption gastrointestinal tract ecosystem, formed by microbiom...

Background: The characteristics and roles of gut lymphocytes have been only partly elucidated, in particular with regard to activation patterns. Objectives: To characterize lymphocytes from various parts of the gut and examine their activation pattern as a network. Methods: Lymphocytes were isolated from the epithelium, the lamina propria, Peyer's patches, mesenteric lymph nodes, the spleen, an...

The simple quantitation of intraepithelial lymphocytes per 100 epithelial cells in small bowel has been a valuable addition to the study of the histopathology of celiac disease. However new technology is necessary to increase the accuracy and significance of the subtypes of intraepithelial lymphocytes in the differential diagnosis and the evaluation of gluten related disorders.

Highly purified populations of lymphocytes were obtained from the murine intestinal mucosa using EDTA-collagenase isolation procedures in combination with discontinuous density centrifugation. Intraepithelial lymphocytes (IEL) were separated from lamina propria lymphocytes (LPL) and, within these two populations, fractions enriched or depleted in gut granular lymphocytes (gGL) were obtained. Us...

It is intuitive that immune cells in the gut may require microbiota-derived cues for their differentiation. The proximity between host and microbe in the intestine would seemingly necessitate co-adaptation. However, it has been challenging to determine the members and features of the gut microbiota that influence immune system development and function. The recent identification of immunomodulat...

The lymphocytes of epithelial and lamina proprial compartments of the intestine are phenotypically and functionally distinct and serve a wide range of functions in the intestinal mucosa like regulating intestinal homeostasis, maintaining epithelial barrier function as well as regulating adaptive and innate immune responses. To analyze the role of these cells in different disease states, it is n...