OBJECTIVE To investigate the feasibility of Descemet stripping automated endothelial keratoplasty (DSAEK) using cultured human corneal endothelial cells (HCECs) in an animal model. METHODS Descemet stripping automated endothelial keratoplasty grafts were produced by seeding cultured HCEC suspensions onto human corneal stromal discs. Three insertion techniques were assessed in an ex vivo model...

purpose: to evaluate endothelial cell density (ecd) and morphology of clear corneal grafts after descemet stripping automated endothelial keratoplasty (dsaek) and to determine the factors that influence graft ecd postoperatively. methods: seventy-seven eyes (38 right eyes) of 64 patients (34 female subjects) who underwent dsaek for fuchs’ endothelial dystrophy (n=38) or pseudophakic bullous ker...

purpose: to compare corneal biomechanical properties and intraocular pressure (iop) in eyes with pseudophakic bullous keratopathy (pbk) before and after descemet stripping automated endothelial keratoplasty (dsaek). methods: this prospective non-randomizedintrasubject comparative study was conducted on 44eyes of 22 patients with the diagnosis ofpbk who underwent dsaek in one eye.intraocular pre...

Descemet-stripping automated endothelial keratoplasty (DSAEK) has become the procedure of choice to treat corneal endothelial dysfunction. The technique involves replacing the diseased host endothelium with a graft consisting of a thin layer of posterior stroma, Descemet membrane, and endothelium. In comparison to penetrating keratoplasty (PK), DSAEK confers quicker visual and structural recove...

Department of Ophthalmology, Sir Run Run Shaw Hospital, Zhejiang Univ Abbreviations and Acronyms: CDS 1⁄4 Cornea Donor Study; DMEK 1⁄4 Descemet membrane endothelial keratoplasty; DSEK 1⁄4 Descemet stripping endothelial keratoplasty; ECD 1⁄4 endothelial cell density; EK 1⁄4 endothelial keratoplasty; FECD 1⁄4 Fuchs’ endothelial corneal dystrophy; MCMC 1⁄4 Markov chain Monte Carlo; PACE 1⁄4 pseudo...

Title: Spontaneous Reattachment of Dislocated Endothelial Graft After non-Descemet Stripping Automated Endothelial Keratoplasty: A Case Report Authors: Aya Kodama-Takahashi ([email protected]) Masahiko Fukuda ([email protected]) Koji Sugioka ([email protected]) Akira Kobayashi ([email protected]) Yoshikazu Shimomura ([email protected]) Version: 1 Date: 21 Dec 2017 Au...