نتایج جستجو برای: dependent probe amplifi cation
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L, LT, LTC, LTM, Linear Technology and the Linear logo are registered trademarks of Linear Technology Corporation. All other trademarks are the property of their respective owners. A great deal of progress has been made in op amp DC characteristics. Carefully executed designs currently available provide sub-microvolt VOS ΔT drift, low bias currents and open-loop gains exceeding one million. Con...
To avoid false positive Polymerase Chain Reaction (PCR) detection, caused by cross-contamination with amplicons from earlier experiments, deoxyuridine triphosphate (dUTP) is commonly used as a substitute for deoxythymidine triphosphate (dTTP) in PCR mastermixes. This allows the performance of a decontamination step prior to PCR amplifi cation. Analysis of PCR products on the Agilent 2100 Bioana...
Death rates from hepatocellular carcinoma (HCC) are steadily increasing, yet therapeutic options for advanced HCC are limited. We identify a subset of mouse and human HCCs harboring VEGFA genomic amplifi cation, displaying distinct biologic characteristics. Unlike common tumor amplifi cations, this one seems to work via heterotypic paracrine interactions; stromal VEGF receptors (VEGFR), respond...
An effi cient method for RNA extraction that leads to RNA high yield and purity is a technical issue relevant for development and optimization of molecular diagnostic methods aimed to detect viroid infections in citrus varieties. Residual contaminants may affect RNA detection depending on the molecular diagnosis approaches. This condition can be evaluated through RNA absorption spectrum analysi...
Today’s modern commercial and military communication systems are demanding better performance with regards to power, effi ciency, linearity and operating bandwidth. As such, extra considerations must be placed on designing the internal components of the systems, including the low-noise and power amplifi ers, to maximize performance. In order to reach higher effi ciencies, signifi cant research ...
The genus Eucalyptus encompasses several species of industrial importance. Many of these species have been subjected to genetic characterization using different kinds of DNA markers. More than 1000 microsatellites have been identifi ed from the genome of eucalypts and they are highly amenable for cross species transferability. During cross amplifi cation of microsatellites, homoplasy is reporte...
A fl uorescence sensor array is being developed to detect explosives at ambient temperature and atmospheric conditions. The sensor platform uses a multilayer substrate that serves to amplify the emission signal by more than three orders of magnitude, improving the sensor response accordingly. Studies to understand the mechanism of the signal amplifi cation are being undertaken so that the senso...
To the Editor: Bartonella alsat-ica was fi rst isolated from the blood of wild rabbits from the Alsace region in France (1). This bacterium is now considered an emerging infectious disease zoonotic agent in persons in close contact with rabbits; at least 2 human cases of endocarditis and 1 human case of lymphadenitis have been reported (2–4). In this study, we report the molecular detection of ...
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