Considering the ease of isolation and high expansion potential of pulp stem/progenitor cells isolated from wisdom and primary teeth they have been implicated as the most reliable autologous cell source in dental tissue engineering. Meanwhile, different isolation methods have remarkable impacts on the expansion potential of adult stem cells. In enzymatic digestion method extracted teeth as denta...

introduction: nanoparticles are being increasingly applied in dentistry due to their antimicrobial and mechanical properties. this in vitro study aimed to assess and compare the cytotoxicity of four metal oxide nanoparticles (tio 2 , sio 2 , zno, and al 2 o 3 ) on human dental pulp stem cells. methods and materials: four suspension with different concentrations (25, 50, 75, 100 µg/ml) of each n...

Introduction Based on previous researches, dental pulp stem cells (DPSCs) are easily accessible with limited morbidity after collection. Their embryonic origin, from neural crests, explains their multipotency. DPSCs are primarily derived from the pulp tissues of the teeth.Objective: This study was undertaken to isolate, culture, and characterize two different third molar and first premolar hum...

Dental pulp tissue was collected from 6 healthy adult patients, prior to prosthetic treatments, in order to evaluate the in situ phenotype of dental pulp stromal cells and compare with that of dental pulp stem cells. A CD34-/CD44+/CD105-/CD117+/CD146-/nestin- phenotype of stromal cells in the dental pulp core was found. Cells with a similar phenotype, but CD44-, were found in the cell richzone....

Regeneration of dental pulp after pulpectomy was accomplished recently by autologous transplantation of dental pulp stem/ progenitor cells into the root canal. The identical patterns of qualitative and quantitative protein and mRNA expression in the regenerated pulp and normal pulp demonstrated complete pulp regeneration. The lack of tissue specific markers in the dental pulp is a major challen...

Background: It is still controversial that the stem cells isolated from human dental pulp meets the criteria for mesenchymal stem cells (MSCs). The aim of the present study was to examine whether or not they are MSCs, or are distinct stem cells population residing in tooth pulp. Methods: Adherent fibroblastic cells in the culture of pulp tissue from human third molars were propagated through se...

Introduction: During the last decade, advances in tissue engineering and stem cell-based tooth regeneration have provided realistic and attractive means of replacing lost or damaged teeth. The first adult stem cells isolated from dental tissues were dental pulp stem cells (DPSCs). When transplanted with hydroxyl apatite/tri calcium phosphate (HA/TCP) powder, they formed a dentin-like structure...

Interactions between ingrowing nerve fibers and their target tissues form the basis for functional connectivity with the central nervous system. Studies of the developing dental pulp innervation by nerve fibers from the trigeminal ganglion is an excellent example of nerve-target tissue interactions and will allow specific questions regarding development of the dental pulp nerve system to be add...

background: it is still controversial that the stem cells isolated from human dental pulp meets the criteria for mesenchymal stem cells (mscs). the aim of the present study was to examine whether or not they are mscs, or are distinct stem cells population residing in tooth pulp.   methods: adherent fibroblastic cells in the culture of pulp tissue from human third molars were propagated through ...