BACKGROUND In 1951, Ardran reported that metastatic bone lesions could be detectable on plain radiography with 30% to 50% of decalcification. Authors performed experimental study for minimum level of decalcification to detect the osteolytic bone metastasis of long bone with recent technique of radiographs. METHODS One pair of fibula and humerus from two cadavers was cut into specimen 1 inch i...

1. In all age groups the effects of ammonium chloride administration were found to be strikingly dependent upon the calcium intake. 2. Dogs receiving an adequate calcium diet and ammonium chloride showed less decalcification than those receiving a low calcium diet with or without ammonium chloride. 3. In the younger groups the added effect of ammonium chloride to calcium-low diet brought out mo...

Abstract The resistance of alkali-activated materials (AAMs) to degradation processes, particularly the decalcification, was studied in this paper. ground granulated blast furnace slag using various activators with same activator dosage 6% Na 2 O by weight (sodium hydroxide, sodium waterglass and carbonate) subjected testing decalcification (immersion 6M NH 4 NO 3 ) for 84 days. reference sampl...

A total of 78 orthodontic patients participated in a study of the degree of decalcification inhibition induced by daily treatment with 0.4% SnF2 gel. Control patients (n = 39) were managed exactly as were the test patients (n = 39) except that the latter were instructed to brush at bedtime each day with the gel. In the nongel users, 24 (64.1%) developed decalcification after 18-24 months bandin...

To facilitate the molecular analysis of tissues in adult zebrafish, we tested eight different fixation and decalcification conditions for the ability to yield DNA suitable for PCR and tissue immunoreactivity, following paraffin embedding and sectioning. Although all conditions resulted in good tissue histology and immunocytochemistry, only two conditions left the DNA intact as seen by PCR. The ...

Bone decalcification is a time-consuming process. It takes weeks and preservation of the tissue structure depends on the quality and velocity of the demineralization process. In the present study, a decalcification methodology was adapted using microwaving to accelerate the decalcification of rat bone for electron microscopic analysis. The ultrastructure of the bone decalcified by microwave ene...

This note reports a method for studying the relationship between crystals and the organic matrix in calcified structures by means of decalcification of single thin sections. The mollusc shell has been used in developing the method. Electron microscope studies of the organic matrix of the mollusc shell have been carried out by making replicas of the normal or etched shell surfaces (Gr5goire, 195...

Decalcification is commonly employed in most histopathology laboratories for the microscopical examination of bone and other calcified tissues. Plastic processing without decalcification may produce superior results in terms of eliminating shrinkage and for demnonstrating osteoid versus mineralised matrix'-' but may give poor cytological detail and is a much longer process. The diagnosis of non...