The aim of this study is to investigate the production of bio-hydrogen, via dark anaerobic fermentation in a pilot scale reactor under batch conditions, using a mixed microflora. As inoculum, anaerobic digested sludge, collected from municipal wastewater treatment plant, was used after being pre-treated with HCl 1N for 24h (pH 3) in order to inhibit the methanogenic bioactivity. As a source of ...

Food waste and food processing wastes which are abundant in nature and rich in carbon content can be attractive renewable substrates for sustainable biohydrogen production due to wide economic prospects in industries. Many studies utilizing common food wastes such as dining hall or restaurant waste and wastes generated from food processing industries have shown good percentages of hydrogen in g...

Harnessing hydrogen competently through wastewater treatment using a particular class of biocatalyst is indeed a challenging issue. Therefore, biohydrogen potential of real-field wastewater was evaluated by hybrid fermentative process in a single-stage process. The cumulative hydrogen production (CHP) was observed to be higher with distillery wastewater (271 mL) than with dairy wastewater (248 ...

In this study, the IWA anaerobic digestion model N°1 (ADM1) was used to simulate and predict the dark fermentation process of a complex organic substrate: household waste. Since ADM1 does not account for metabolic products such as lactic acid, ethanol and some volatiles fatty acids like caproate, that appear during the dark fermentation process, the structure of ADM1 was modified to include the...

This study determined hydrogen production, volatile fatty acids (VFAs) generation and cellulose solubilisation from anaerobic dark fermentation of wheat stalk and showed the effect of different mixed microflora. The cumulative hydrogen yields of anaerobic digested activated sludge (AS)-inoculated and anaerobic digested dairy manure (DM)-inoculated system were 23.3 and 37.0 mL/g VS at 204 h, res...

1.. After 2 hours of fermentation in nitrogen the metabolism of those algae which were found capable of photoreduction with hydrogen changes in such a way that molecular hydrogen is released from the cell in addition to carbon dioxide. 2. The amount of hydrogen formed anaerobically in the dark depends on the amount of some unknown reserve substance in the cell. More hydrogen is formed in presen...

Hydrogen gas production from cellulose was investigated using an integrated hydrogen production process consisting of a dark fermentation reactor and microbial fuel cells (MFCs) as power sources for a microbial electrolysis cell (MEC). Two MFCs (each 25 mL) connected in series to an MEC (72 mL) produced a maximum of 0.43 V using fermentation effluent as a feed, achieving a hydrogen production r...

Biohydrogen production (BHP) can be achieved by direct or indirect biophotolysis, photo-fermentation and dark fermentation, whereof only the latter does not require the input of light energy. Our motivation to compile this review was to quantify and comprehensively report strains and process performance of dark fermentative BHP. This review summarizes the work done on pure and defined co-cultur...

What if it was mentioned that there are good microbes can eat garbage? There may be reports stating bad and cause diseases. However, also the organic garbage (like banana peels old vegetables) generated in houses or restaurants. These use to produce energy, form of hydrogen, which used fuel our cars. The process turning into energy is called dark fermentation. During fermentation, other helpful...