interaction of glucose 6-phosphate dehydrogenase from s. aureofaciens with nad+, nadp+and glucose 6-phosphate were investigated using different fluorescent probes. binding of nad+, nadp+and s-nadph to the native enzyme quenched intrinsic protein fluorescence by 100%, 10% and 21%,respectively, from which kd values of nad+ (6.5 mm), nadp+ (92.0 μm) and s-nadph (122.0 μm)were calculated. binding o...

The Research Laboratories of the Upjohn Company, Kalamazoo, Michigan, U.S.A., have isolated a new antimicrobic complex pro­ duced under conditions of submerged fermentation by Streptomyces spectabilis, an Actinomycete isolated from a sample of soi! from Dallas, Texas, U.S.A. The laboratory designation of Antibiotic 10la bears the generic na me Streptovaricin and ha s subsequently been marketed ...

calcium acetate is used as an oral phosphate binder to control hyperphosphatemia in patients with chronic renal failure. compared to calcium carbonate, control of hyperphosphatemia can be achieved at lower calcium administration with calcium acetate which likely reduces the risk of hypercalcemia. in this study, various formulations of calcium acetate tablets were prepared and their disintegrati...

inoculation of phosphate-solubilizing microorganisms along with rock phosphate is known to enhance the available p from soil. the success of such solubilization is largely dependent on the ability of the inoculant strain to survive under adverse environmental conditions. in this context, liquid inoculants are gaining importance and are becoming popular with longer shelf-life. in the present inv...

some kinetic properties of nad+- and nadp+- dependent glucose 6-phosphatedehydrogenase (g6pd) purified from streptomyces aureofaciens were studied. both nadh andnadph inhibited the enzyme competitively and noncompetitively, with respect to the correspondingcoenzymes and glucose 6-phosphate, respectively. atp inhibited the nad+ - linked reaction but not thatof the nadp+- linked activity. the inh...

the voltammetric behaviour of sulfadoxine (sdn) was studied at a glassy carbon electrode in 0.2 m phosphate buffer solutions using cyclic, differential-pulse (dpv) and square wave voltammetry (swv). the dependence of the current on ph, concentration, and scan rate was investigated to optimize the experimental conditions for the determination of sdn. the oxidation process was shown to be diffusi...

in order to evaluate the effect of balanced application of chemical phosphorus fertilizer and seed inoculation with phosphate solubilizing bacterium and arbuscular mycorrhizal fungus and on the yield and yield components of maize (zea mays l.) under normal and limited irrigation conditions, a split-split plots arrangement based on randomized complete block design with three replications was con...