The biological effects of static magnetic fields, and their combined effects with ionizing radiation, were studied using a cultured mammalian cell line (FM3A). The three different modes of magnetic fields evaluated in this report were the 0.3 Tesla (T) field with a gradient of 0.3T/m, the 0.7T field with a gradient of 0.7T/m and the 6.34T field with no gradient. Exposure to the 0.3T and 0.7T fi...

The epidermal growth factor receptor (EGFR) plays an important role in neoplastic growth control of malignant gliomas. We have demonstrated that radiation activates EGFR Tyr-phosphorylation (EGFR Tyr-P) and the proliferation of surviving human carcinoma cells, a likely mechanism of accelerated cellular repopulation, a major cytoprotective response after radiation. We now investigate the importa...

In 2005, a human adenovirus strain (formerly known as HAdV-D22/H8 but renamed here HAdV-D53) was isolated from an outbreak of epidemic keratoconjunctititis (EKC), a disease that is usually caused by HAdV-D8, -D19, or -D37, not HAdV-D22. To date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species Hum...

Six bacterial isolates from the U.S. DOE Subsurface Science Program and three reference bacteria were tested for resistance to UV light and gamma radiation. The subsurface isolates included three aerobic, pigmented, Gram positive bacteria and three microaerophilic, non-pigmented, Gram negative bacteria. Deinococcus radiodurans was the most resistant bacterium to both types of radiation, with a ...

Background: Human Adenovirus species D (HAdV-D) was common human viral pathogen especially in eye infection, consists of several types of which HAdV-D8, -D19 and –D37 were common in eye infection. This study includes detection of HAdV-D types implicated in conjunctivitis based on L2 (Penton protein) gene similarity. Methods: Conjunctival swabs were collected from Keratoconjunctivitis patient...

Lethal effects of 60Co gamma-rays, UV light, and mitomycin C on two kinds of bacteria, Halobacterium salinarium which grows in highly concentrated salt media and Thiobacillus intermedius which requires reduced sulfur compounds, were studied and compared with those on Escherichia coli B/r. D37 values for H. salinarium, T. intermedius and E. coli B/r were 393, 150, and 92 Gy, respectively, by exp...

Data for the inactivation of three Escherichia coli mutants by energetic heavy ions are fitted by the track theory of a one-hit detector in an extended target mode. The respective E0's are 46, 36.5, and 12.6 Gy for E. coli B, B/r, and Bs-1 and a0, the assumed target radius, is 0.5 microns for all three. The parameter E0, the D37 with gamma rays, is measured directly, while a0 is fitted to the d...

target of radiation action. The greatest toxicity is Purpose : The incubation of the DNA minor-groove binder observed when the I atom is incorporated within [I]iodoHoechst 33342 (IH) with plasmid DNA leads to the the DNA helix (i.e. 5-[I]iodo-2 3⁄4 -deoxyuridine, production of one double-strand break (dsb) per decay, both in IdUrd (Kassis et al. 1988)) , while its dislocation by the presence an...

SUMMARY A procedureis describedwherebyshortenedDNA single strands can be selectively eluted from filters upon which cells have been lysed. This provides a sensitive meas ure of DNA single-strand breakage that may be applicable to the detection of cell damage resulting from.certain types of chemotherapeutic agents. The procedure was applied to the following processes in L12l0 cells: (a) producti...