Transfer of Cytoplasmic Male Sterility into Rice Diversifying the existing cytoplasmic male sterility (CMS) sources of rice is indispensable for a sustainable production system of hybrid seed. Ahmadikhah et al. report that maintainer line Yosen B was converted to a CMS line (Yosen A) in three generations by markerassisted backcrossing. The recurrent parent genome content of the final CMS line w...

Human RNA methyltransferase BCDIN3D is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. Here, we show that cytoplasmic tRNAHis is the primary target of BCDIN3D in human cells. Recombinant human BCDIN3D, expressed in Escherichia coli, monomethylates the 5΄-monophosphate of cytoplasmic tRNAHis efficiently in vitro. In BCDN3D-kn...

Cytoplasmic control of preimplantation development is not a 'new' concept; the first cytoplasmic transfer experiment was performed in the mouse during the early 1980s, as a means of overcoming cleavage arrest at the 2-cell stage, the '2-cell block'. Since the first human pregnancy following the transfer of cytoplasm from donor oocytes into the oocytes of a patient with a history of poor embryo ...

Transmembrane location of the retinal chromophore, either native or reduced in situ to a fluorescent derivative, of the purple membrane of Halobacterium halobium was investigated with fluorescence energy transfer techniques. Single sheets of purple membrane, either native or reduced with borohydride, were adsorbed on polylysine-coated glass; the orientation, whether the exposed surfaces were cy...

background: c-reactive protein (crp) can be increased after hormonal stimulations. the changes of crp might affect the success of in-vitro fertilization (ivf). objective: the aim of this study was to determine the possible relationship between the serum crp level and outcome of controlled ovarian stimulation, and pregnancy rate in patients undergoing ivf or intra cytoplasmic sperm injection (ic...

background: embryo transfer (et) has traditionally been performed two days after oocyte retrieval. delaying transfer from day two to day three or four would allow for further development of the embryo might therefore optimize the selection of viable and best quality embryos for transfer and may be closer to the physiological time of the entry into the uterus than transfer on day two and might h...

Recent evidence from different research areas has revealed a novel mechanism of cell-cell communication by spontaneous intercellular transfer of cellular components (ICT). Here we studied this phenomenon by co-culturing different cells that contain distinct levels of proteins or markers for the plasma membrane or cytoplasm. We found that a variety of transmembrane proteins are transferable betw...