viewed as a signal transduction messenger. We present herein the cytochemical approach for detecting NADPH oxidase actMty in human neutrophils under both an electron microscope and a CCD camera-equipped fluorescence microscope, The applications of the cytochemical methodology are also ment;oned in this review. EspeciaHy. the following mechanisms for the release of superoxide from human neutroph...

Human peripheral blood lymphocytes that express T cell markers, when reacted for the cytochemical localization of lysosomal acid hydrolases, display two major patterns of staining, i.e., dot-like and scattered granular. Previous attempts to fractionate T cells according to surface markers have yielded populations of cells with heterogeneous patterns of cytochemical staining. In this study, peri...

Alterations for acid and alkaline phosphatase levels and their pattern of splenic and lymph node activity in normal and virus-induced lymphoblastic leukemia were studied. Enzyme levels were examined by using both cytochemical and biochemical procedures. The GC leukemia virus, a ribonucleic acid murine virus antigenically related to the Rauscher-Moloney viruses, was used to stimulate acid and al...

The aggressive clinical course and the distinctive histologic, cytochemical, and cytogenetic features of an adult non-Sézary T-cell lymphoma with suppressor activity have been investigated. Morphological and ultrastructural analysis of neoplastic cells from peripheral blood and involved lymph nodes revealed cells with convoluted nuclei, prominent cytoplasmic azurophilic granules, well developed...

The purpose of our study was to determine the effect of dilution and liquid-preservation of boar sperm on oxidoreductive capability of their mitochondria. The semen was diluted with BTS extender produced from water purified by destillation or by reverse osmosis. The spermatozoa were stored over a four-day period at 16-18 degrees C. The function of sperm mitochondria was assessed using the scree...

Niemann-Pick disease type C (NP-C) is a neurovisceral lysosomal cholesterol trafficking and lipid storage disorder caused by mutations in one of the two genes, NPC1 or NPC2. Diagnosis has often been a difficult task, due to the wide range in age of onset of NP-C and clinical presentation of the disease, combined with the complexity of the cell biology (filipin) laboratory testing, even in combi...

We have found that alkaline phosphatase, an enzyme often associated with active transport, is present in the contractile vacuole of the ameba, Acanthamoeba castellanii . Earlier biochemical studies (1, 2) had suggested that this enzyme and a 5'-nucleotidase might be associated with the plasma membrane . Highly purified plasma membranes were isolated from Acanthamoeba by Schultz and Thompson (I)...