in this study the programmed cell death (apoptosis) of human neutrophilic granulocytes was investigated in the presence and absence of fetal calf serum (fcs), cycloheximide and actinomycin-d. the results show that when fcs is omitted from cultures, apart from a decrease in viability, the percentage of apoptotic cells and dna fragmentation increases. apoptosis is accelerated in serum withdrawal ...

1. Effects of short-term and long-term administration of cycloheximide on rat liver mitochondrial protein synthesis have been examined and were found to be different. 2. Long-term administration of cycloheximide resulted in inhibition of total cellular protein synthesis including that of mitochondria while, at short-term intervals, 8 10 % of mitochondrial protein synthesis was cycloheximide-res...

Cycloheximide (3 to 5 mM) caused a decrease in the rate of gluconeogenesis from pyruvate, lactate, or glycerol in the perfused rat and guinea pig liver. In these experiments, oxygen consumption was reduced by 30% with pyruvate but was unaltered with lactate or glycerol as substrates. The inhibition of gluconeogenesis caused by cycloheximide was rapid in onset (lOOrrj, of maximal inhibition in 1...

Pig oocytes at metaphase II were activated by penetration of spermatozoa in cycloheximide-free and cycloheximide-containing fertilisation media. The precise nuclear stage, and the kinetics of degradation of cyclin B1 and dephosphorylation of MAP kinase were assessed after insemination. After maturation culture, 96% of oocytes reached metaphase II. At 6 h after insemination in cycloheximide-free...

Cycloheximide was shown to block abscisic acid (ABA) biosynthesis in nonstressed as well as in stressed Phaseolus vulgaris leaves. Leaf wilting caused by cycloheximide resulted from increased stomatal opening as judged by a decreased stomatal diffusion resistance. The inhibition of ABA biosynthesis by cycloheximide was at least partially responsible for the increase in stomatal opening as sugge...

The early postnatal period represents a time of significant neural development. Neonatal exposure to stressors, environmental toxins, and many different pharmacological agents may have a significant impact on subsequent behavior and cognition in adulthood. The purpose of this study was to examine the effects of early administration of the protein synthesis inhibitor, cycloheximide, on a battery...