Corticotropin-releasing factor (CRF) administered intracerebroventricularly (i.c.v.) activates noradrenergic locus coeruleus (LC) neurons of halothane-anesthetized and unanesthetized rats. This study used a technique for microinfusing CRF into the LC from calibrated micropipettes to characterize and quantify the effects of locally administered CRF on LC discharge in halothane-anesthetized rats....

In this study, plant height and plant dry weight of five wheat varieties (namely Da da 94, K nac 97, Konya 2002, Karahan 99 and Ahmeta a), collected for 20 weeks, were modeled via fitting generalized logistic (GLC), logistic (LC) and Gompertz (GC) growth curves. Growth curve selection was based on residual sum of square (RSS) and mean square error (MSE). Following growth curve selection, growth...

The learning curve (LC) concept has become a dogma in surgery, and also thyroidectomy. This chapter aims to define criteria for the LC most common surgical techniques of thyroidectomy, by investigating number procedures required single surgeon achieve competence parameters used proficiency. Methods calculate each technique (conventional minimally invasive robotic approaches) are discussed analy...

OBJECTIVE To determine the frequency and reasons of conversion of laparoscopic cholecystectomy (LC) to open cholecystectomy (OC) in a tertiary care teaching hospital. METHODS A prospective analysis of conversion of laparoscopic to OC was performed in one Surgical Unit of Civil Hospital Karachi, from 1st September 1997 to 31st May 2005. There were 1238 patients in the series. The inclusion cri...

OBJECTIVE We evaluated the effects of mixed meals differing in glycemic index (GI) and carbohydrate content on postprandial serum glucose and insulin response, hunger, and satiety over the course of a 12-h day. RESEARCH DESIGN AND METHODS In this randomized crossover trial, 26 overweight or obese adults received four diets in random order (high GI, high carbohydrate [HGI-HC]; high GI, low car...

Eight cattle (288 ± 22 kg) were treated with 2.2 mg/kg of body weight of flunixin free acid in a crossover design by subcutaneous (SC) and intravenous (IV) administration. After a minimum 1:10 dilution with 50 mM phosphate buffer, a commercial immunoassay was adapted to determine plasma concentrations of flunixin. The limit of detection was 0.42 ng/mL and the working range was 0.76-66.4 ng/mL w...

A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the determination of donepezil in human plasma samples. Diphenhydramine was used as the internal standard. The collision-induced transition m/z 380 --> 91 was used to analyze donepezil in selected reaction monitoring mode. The signal intensity of the m/z 380 --> 91 transition was found to relate l...

This study tested the hypothesis that endogenous glucocorticoids regulate a putative neurotransmitter function of corticotropin-releasing factor (CRF) in the locus coeruleus (LC). LC spontaneous discharge and activation by intracerebroventricularly administered CRF, hypotensive challenge, sciatic nerve stimulation, and carbachol were compared in adrenalectomized and sham-operated halothane-anes...

UNLABELLED The lumped constant (LC) is a correction factor used to infer glucose metabolic rate (MR(glc)) from FDG metabolic rate (MR(FDG)). METHODS LC was determined in normal brain in 10 subjects (4 male, 6 female) by measuring regional MR(glc) and MR(FDG) independently using 1-(11)C-glucose and (18)F-FDG with dynamic positron tomographic imaging, arterial blood sampling, and region-of-inte...

We investigated if whether intrauterine protein restriction in combination with overfeeding during lactation would cause adult-onset obesity and metabolic disorders. After birth, litters from dams fed with control (17% protein) and low protein (6% protein) diets were adjusted to a size of four (CO and LO groups, respectively) or eight (CC and LC groups, respectively) pups. All of the offspring ...