Hydrogels with predictable degradation are highly desired for biomedical applications where timely disintegration of the hydrogel (e.g., drug delivery, guided tissue regeneration) is required. However, precisely controlling hydrogel degradation over a broad range in a predictable manner is challenging due to limited intrinsic variability in the degradation rate of liable bonds and difficulties ...

Mycoplasma contamination of cell cultures is a serious and widespread problem. Contaminated cultures are frequently discarded or the contamination ignored since treatment with standard antibiotics is undesirable, time-consuming, and an ineffective means to eliminate mycoplasmas. Minerva Biolabs developed an innovative methode based on the biological reagent Mynox. A single treatment over one pa...

We show that the disintegration operator on a complete separable metric space along a projection map, restricted to measures for which there is a unique continuous disintegration, is strongly Weihrauch equivalent to the limit operator Lim. When a measure does not have a unique continuous disintegration, we may still obtain a disintegration when some basis of continuity sets has the Vitali cover...

In order to quantitate lymphocyte proliferative responses, we explored the role of cell death in the kinetics of phytohemagglutinin-stimulated cultures. Unless the disintegration time (tDIS) of nonviable lymphocytes in culture is known, the rate of cell death cannot be calculated. To obtain tDIS, we determined the time interval between total and viable cell population decay after various killin...

The bacillus of glanders may be so modified in virulence as to produce experimentally lesions differing widely in their histological features. The highly virulent culture causes primary necrosis and disintegration of the tissue followed by the invasion of the injured area by polymorphonuclear leucocytes. The bacilli of moderate virulence give rise to a primary lesion of an acute inflammatory na...

Most molecular fingerprinting techniques, including denaturing gradient gel electrophoresis (DGGE) [1], comparative genomic hybridization (CGH) [2], real-time polymerase chain reaction (RT-PCR) [3], destroy community structure and/or cellular integrity, therefore lost the info. of the spatial locus and the in situ genomic copy number of the cells. An alternative technique, fluorescence in situ ...

The purpose of the research is to comprehend highly polymorphic notion identity in works Anna Marie McLemore, a Mexican American writer. A detailed inspection his indicates that concept ‘identity disintegration’ concern virtually all them. This aims demonstrate development as process rather than distinct result struggle. Identity development, an ever-changing process, provides us with new avenu...

The problem of controlling cylindrical tank bioreactor conditions for cell and tissue culture purposes has been considered from a flow dynamics perspective. Simple laminar flows in the vortex breakdown region are proposed as being a suitable alternative to turbulent spinner flask flows and horizontally oriented rotational flows. Vortex breakdown flows have been measured using three-dimensional ...

The disintegration of recovered paper is the first operation in the preparation of recycled pulp. It is known that the defibering process follows a first order kinetics from which it is possible to obtain the disintegration kinetic constant (KD) by means of different ways. The disintegration constant can be obtained from the Somerville index results (%ISV) and from the dissipated energy per vol...