conclusions these results indicate that a. kellalensis extracts exert potent anti-rotaviral activity only after viral adsorption. the two extracts from a. kellalensis showed a good selectivity index. also these results suggest that extracts prepared from the flowers of a. kellalensis may be potential anti-rotaviral agents in vivo and be useful in veterinary medicine. background achillea kellale...

objective(s):bacterial biofilm has been considered responsible for many deaths and high health costs worldwide. their better protection against antibacterial agents compared to free living cells leads to poor treatment efficiency. nanotechnology is promising approach to combat biofilm infections. the aim of the present study was to eradicate staphylococcus epidermidis biofilm with silver nanopa...

AIMS  To develop a novel Vero cell assay that implements a real-time cell electronic sensing (RT-CES) system for the determination of the presence of verotoxin-producing Escherichia coli (VTEC). The assay overcomes the major drawbacks in conventional Vero cell assay, for example, labour-intensive and time-consuming. METHODS AND RESULTS  Cells were grown onto the surfaces of microelectronic se...

Twenty four reference strains and 112 clinical isolates of coagulase negative staphylococci (CNS) were examined for their reactions in the crystal violet test. Some species gave a white reaction and others a purple reaction. Results were consistent and reproducible and each species gave only one pattern of crystal violet reaction. Within the limited variety of species represented in the clinica...

A rapid, cytopathic effect reduction assay for human interferon (IFN) is described. Dilutions of IFN were made with an automated diluter in 96-well microtiter plates. Total incubation time was 26 h. IFN titers were calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, which required less than 1 min to read each plate.

A G-quadruplex-based, label-free, switch-on fluorescence detection method has been developed for the selective detection of ATP in aqueous solution using crystal violet as a G-quadruplex-selective probe. The assay is highly simple and rapid, and does not require the use of fluorescent labeling.

Several studies have validated the effectiveness of narrow-band imaging (NBI) in estimating invasion depth of early colorectal cancers. However, comparative diagnostic accuracy between NBI and chromoendoscopy remains unclear. Other than crystal violet, use of acetic acid as a new staining method to diagnose deep submucosal invasive (SM-d) carcinomas has not been extensively evaluated. We aimed ...

Bacterial colonization of polymeric medical aids causing so-called nosocomial infections is one of the main problems linked with the extensive use of polymeric devices in clinical applications. In this study, the properties of medical-grade polyvinyl chloride were modified by incorporating crystal violet into its bulk structure. Sodium montmorillonite was used as a carrier for the crystal viole...

A series of aminotriarylmethane dyes were examined for binding to the nicotinic acetylcholine receptor (AChR) from Torpedo californica. Several compounds were found to bind to the noncompetitive antagonist site of the AChR as demonstrated by inhibition of [3H]phencyclidine binding; apparent KD values ranged from 50 nM to >100 microM. One dye with high affinity, crystal violet, revealed a greate...

Borzani and Vairo (1959) reported that grampositive and gram-negative microorganisms are fundamentally different with respect to crystal violet adsorption. They claimed that the binding of crystal violet by gram-positive cells followed the Freundlich and the Langmuir adsorption laws; whereas the binding of crystal violet by gram-negative cells did not follow any known adsorption law. The presen...