Objective Oocyte cryopreservation is an essential part of assisted reproductive technology (ART) which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems (Cryotop and Open Pulled Straw, OPS) on mature oocytes gene expressions. MaterialsAndMethods The survival rate of metaphase II (MII) mouse oocytes were assessed after cryoprese...

In vitro maturation of vitrified immature germinal vesicle (GV) oocytes is a promising fertility preservation option. We analyzed the ultrastructure of human GV oocytes after Cryotop vitrification (GVv) and compared it with fresh GV (GVc), fresh mature metaphase II (MIIc) and Cryotop-vitrified mature (MIIv) oocytes. By phase contrast microscopy and light microscopy, the oolemmal and cytoplasmic...

Two experiments were performed to develop a method to cryopreserve MII human oocytes. In the first experiment, three vitrification methods were compared using bovine MII oocytes with regard to their developmental competence after cryopreservation: (i) vitrification within 0.25-ml plastic straws followed by in-straw dilution after warming (ISD method); (ii) vitrification in open-pulled straws (O...

OBJECTIVE To compare the mouse oocyte vitrification outcomes of the CryoLogic vitrification method (CVM) and the conventional open method using a Cryotop. Two CVM methods (original CVM and modified CVM) were tested. METHODS Mature oocytes obtained from female BDF-1 mice were vitrified by two-step exposure to equilibrium and vitrification solutions. Three vitrification protocols were tested on...

Abstract Background Vitrification has become the method of choice for cryopreservation human embryos and gametes. There are multiple commercial media, containing different combinations concentrations cryoprotectants, available vitrification warming procedures. The aim this retrospective study was to compare post-warming survival rate clinical outcomes cleavage stage vitrified/warmed using two m...

BACKGROUND Oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. The present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by Cryotop method. METHOD A total of 200 germinal vesicle (...

STUDY QUESTION Can the equilibration steps prior to embryo vitrification be automated? SUMMARY ANSWER We have developed the 'Gavi' system which automatically performs equilibration steps before closed system vitrification on up to four embryos at a time and gives in vitro outcomes equivalent to the manual Cryotop method. WHAT IS KNOWN ALREADY Embryo cryopreservation is an essential componen...

conclusions the present study showed that vitrification using cryotop and freezing medium can damage oocytes by reducing the maturation and fertilization rates in both developmental stages. results vitrification caused a significant reduction in the maturation rate of oocytes. of those that matured, the fertilization rate of vitrified ivm-mii (44.1%) and ov-mii oocytes (50%) was not significant...