نتایج جستجو برای: cryopreserving

تعداد نتایج: 96  

2017
Huanhua Chen Guixue Feng Bo Zhang Hong Zhou Jinhui Shu Xianyou Gan

The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was perfor...

Journal: :BioTechniques 2005
Carol B Ware Angelique M Nelson C Anthony Blau

A significant obstacle to using human embryonic stem cells (hESCs) arises from extremely poor survival associated with freezing, typically in the range of 1%. This report describes a slow controlled-rate freezing technique commonly used for mammalian embryo cryopreservation. Using a combination of surviving colony number and colony diameter; survival was determined relative to untreated hESCs. ...

2001
R. A. LEOPOLD W. B. WANG D. R. BERKEBILE T. P. FREEMAN

A method for cryopreserving embryos of the screwworm, Cochliomyia hominivorax (Coquerel), was developed for the long-term storage of strains used in research projects and for colonies maintained as back-up to production strains that are mass-reared for sterile insect release programs. This protocol, when combined with a previous procedure that was designed to render screwworm embryos permeable ...

2017
R. A. Leopold Dennis Berkebile R. A. LEOPOLD W. B. WANG D. R. BERKEBILE T. P. FREEMAN

A method for cryopreserving embryos of the screwworm, Cochliomyia hominivorax (Coquerel), was developed for the long-term storage of strains used in research projects and for colonies maintained as back-up to production strains that are mass-reared for sterile insect release programs. This protocol, when combined with a previous procedure that was designed to render screwworm embryos permeable ...

Journal: :Theriogenology 2008
Phillip H Purdy

The National Animal Germplasm Program (NAGP) is developing a national repository for germplasm (semen, oocytes, embryos, blood, DNA, tissue) for all agricultural species in the US. Currently, the swine collection consists of 127,479 samples from 886 boars representing 20 major, minor and composite populations. Cryopreservation per se is not an impediment to program success. Rather, the greatest...

Journal: :Veterinary medicine and science 2022

Aquaculture is the major aquatic animal production segment. Problems like inbreeding depression, genetic drift, introgressive hybridization, etc. have been influencing of quality seeds negatively. Cryopreservation serves as a way-out for these problems and possible answer to produce genetically improved varieties. It has considered strategy conservation fish resources. gametes in vogue since 19...

Journal: :Indian Journal of Animal Research 2021

Background: Egg yolk (EY) is well known to be toxic for buck spermatozoa, which creates restrictions of its use in cryopreservation. Therefore, this study compare the effect different levels gum Arabic (GA) an extender on quality and fertility cryopreserved sperm. Methods: Each ejaculate six bucks was frozen Tris with one concentrations GA contained 3, 6, 9 12 gm/ 100 ml place EY. Control conta...

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