نتایج جستجو برای: cryopreservation

تعداد نتایج: 7939  

2017

Introduction Causes of impaired female reproductive function Environmental Endocrine Disrupters and Ovarian Function Indications of reproductive function preservation Choice of the method for ovarian function preservation Cryopreservation of Oocytes Introduction The need for oocyte cryopreservation Cryobiological factors influencing cryoefficiency Oocyte structures affected by cryopreservation ...

متن کامل
Journal: :Genetical research 1997
D Houle A S Kondrashov L Y Yampolsky S Caldwell P L Steponkus

Although cryopreservation is routinely used for the storage of a range of biological organisms, few studies have been conducted to determine whether cryopreservation increases the frequency of mutation. A procedure for the cryopreservation of Drosophila melanogaster embryos has recently been developed. Cryopreservation of D. melanogaster is of special interest to geneticists and evolutionary bi...

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2016
Sean M Hughes Zhiquan Shu Claire N Levy April L Ferre Heather Hartig Cifeng Fang Gretchen Lentz Michael Fialkow Anna C Kirby Kristina M Adams Waldorf Ronald S Veazey Anja Germann Hagen von Briesen M Juliana McElrath Charlene S Dezzutti Elizabeth Sinclair Chris A R Baker Barbara L Shacklett Dayong Gao Florian Hladik

BACKGROUND Understanding how leukocytes in the cervicovaginal and colorectal mucosae respond to pathogens, and how medical interventions affect these responses, is important for developing better tools to prevent HIV and other sexually transmitted infections. An effective cryopreservation protocol for these cells following their isolation will make studying them more feasible. METHODS AND FIN...

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Journal: :Bioconjugate chemistry 2011
Mathieu Leclère Bonnie K Kwok Luke K Wu David S Allan Robert N Ben

Significant cell damage occurs during cryopreservation resulting in a decreased number of viable and functional cells post-thawing. Recent studies have correlated the unsuccessful outcome of regenerative therapies with poor cell viability after cryopreservation. Cell damage from ice recrystallization during freeze-thawing is one cause of decreased viability after cryopreservation. We have asses...

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2007
Shigeki OHBOSHI

protectant for spermatozoa41) led to research on cryopreservation of mammalian embryos. In the past quarter of a century since the first successful cryopreservation of mouse embryos58,60), basic and applied research has resulted in the cryopreservation of embryos of many mammalian species. Improvements have been made in nearly all steps of the cryopreservation process, resulting in considerable...

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Journal: :Aesthetic surgery journal 2006
Lee L Q Pu Xiangdong Cui Jihui Li Betsy F Fink Michael L Cibull Dayong Gao

BACKGROUND Successful long-term preservation of adipose tissues may have an important impact on future clinical application of autologous fat transplantation. Our group has recently developed an optimal cryopreservation method for possible long-term preservation of adipose aspirates. OBJECTIVE The purpose of this study was to evaluate the fate of previously cryopreserved adipose aspirates aft...

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Journal: :Annals of clinical and laboratory science 2001
Z Chen Y Ding H Zhang

To determine the best and simplest method for cryopreservation of pig hepatocytes, we compared immediate cryopreservation with cryopreservation after short-term culture. Suckling pig hepatocytes were isolated by a modified 2-step in situ collagenase perfusion method, suspended in serum-free medium, and preserved for 10 da by two cryopreservation methods. Serial measurements were made of cell vi...

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2016
Kenny A Rodriguez-Wallberg Tom Tanbo Helena Tinkanen Ann Thurin-Kjellberg Elizabeth Nedstrand Margareta Laczna Kitlinski Kirsten T Macklon Erik Ernst Jens Fedder Aila Tiitinen Laure Morin-Papunen Snorri Einarsson Varpu Jokimaa Maritta Hippeläinen Mikael Lood Johannes Gudmundsson Jan I Olofsson Claus Yding Andersen

INTRODUCTION The aim of this study is to report the current status of ovarian tissue cryopreservation among alternatives for fertility preservation in the Nordic countries. MATERIAL AND METHODS A questionnaire was sent to 14 Nordic academic reproductive centers with established fertility preservation programs. It covered fertility preservation cases performed up to December 2014, standard pro...

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Journal: :Fertility and sterility 2013
Brooke Hodes-Wertz Sarah Druckenmiller Meghan Smith Nicole Noyes

OBJECTIVE To better understand women's beliefs, priorities, and attitudes toward oocyte cryopreservation, to appreciate the extent of their reproductive education, and to track the reproductive paths of women who chose to undergo oocyte cryopreservation treatment. DESIGN An anonymous 30-question survey. SETTING Not applicable. PATIENT(S) From 2005-2011, 478 women completed ≥1 oocyte cryop...

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Journal: :Philosophical transactions. Series A, Mathematical, physical, and engineering sciences 2010
Feng Xu Sangjun Moon Xiaohui Zhang Lei Shao Young Seok Song Utkan Demirci

Cells and tissues undergo complex physical processes during cryopreservation. Understanding the underlying physical phenomena is critical to improve current cryopreservation methods and to develop new techniques. Here, we describe multi-scale approaches for modelling cell and tissue cryopreservation including heat transfer at macroscale level, crystallization, cell volume change and mass transp...

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