Previous work to elucidate the mechanism of crosslink repair by (A)BC excinuclease has shown that a psoralen-crosslinked duplex is selectively incised in the furan-side strand, while a three-stranded structure is incised in the pyrone-side strand of the crosslink. These observations support a sequential incision and recombination model for the complete error-free repair of a psoralen crosslink....

The optimal process conditions for a high ratio of de-vulcanization to polymer degradation have been elaborated for tire rubbers: SBR, BR, NR and CIIR. These polymers all show their own particular breakdown characteristics, and the temperature dependence of the breakdown mechanism was investigated by measuring sol fractions and crosslink densities. For SBR and BR, the highest reduction in cross...

Applying psoralen interstrand crosslinks for the photoactivation of nucleic acids is a new concept. To find chromophores that can efficiently stimulate crosslink repair we screened several pyrenes and appended them to peptide nucleic acids for their site-selective addressing. Even though pyrenes conjugated to uracil revealed desirable spectroscopic properties they were not effective in crosslin...

The hydroxypyridinium (HP) crosslink is the predominant non-reducible collagen crosslink in heart. HP concentration in left ventricle (LV) increases progressively throughout life, and this increase is thought to reflect the slower turnover of collagenous proteins seen with aging, allowing mature extracellular matrix (ECM) collagen to crosslink more heavily. There are also species differences wi...

SLX4 binds to three nucleases (XPF-ERCC1, MUS81-EME1, and SLX1), and its deficiency leads to genomic instability, sensitivity to DNA crosslinking agents, and Fanconi anemia. However, it is not understood how SLX4 and its associated nucleases act in DNA crosslink repair. Here, we uncover consequences of mouse Slx4 deficiency and reveal its function in DNA crosslink repair. Slx4-deficient mice de...

This paper describes our work in NTCIR-9 on the task of Cross-Lingual Link Discovery (Crosslink/CLLD). The work mainly focuses on two aspects to accomplish this task: (1) How to collect useful data for Crosslink and (2) How to use the data correctly and effectively. The system firstly uses online data collecting and text mining in Chinese Wikipedia articles to build the basic Crosslink database...

Cellular therapies have great potential to provide alternative treatment options for those suffering from heart disease. In order to optimize cell delivery for therapeutic efficacy, a greater understanding of parameters that impact stem cell differentiation, survival, growth, and development are needed. In this study, we examine the role of hydrogel crosslink density on spontaneous cardiomyocyt...

The statistical mechanics of polymers grafted on surfaces has been the subject of intense research activity because of many potential applications. In this paper, we analytically investigate the conformational changes caused by a single crosslink on two ideal (Gaussian) chains grafted onto a rigid planar surface. Both the crosslink and the surface reduce the number of allowed configurations. In...

The response of double-helical DNA to torsional stress may be a driving force for many processes acting on DNA. The 1.55-A crystal structure of a duplex DNA oligonucleotide d(CCAGGCCTGG)(2) with an engineered crosslink in the minor groove between the central guanine bases depicts how the duplex can accommodate such torsional stress. We have captured in the same crystal two rather different conf...

the blending of nr and br rubber blend with n330 carbon black is studied on curing behavior and the physico-mechanical properties of a sbr rubber based tire tread compound containing n234 carbon black. the effect of sulphur and accelerator contents on different properties of rubber compound is also tested with a wide range of curing agents. partial substitution of sbr with nr shows significant ...