This study provides new data on chromosomal characteristics and DNA barcoding of three endemic loaches of Iran: spiny southern loach Cobitis linea (Heckel, 1847), Persian stream loach Oxynoemacheilus persa (Heckel, 1848) and Tongiorgi stream loach Oxynoemacheilus tongiorgii (Nalbant & Bianco, 1998). The chromosomes of these fishes were investigated by examining metaphase chromosome spreads obta...

The genetic structure of 10 populations (453 individuals) of stone loach (Barbatula barbatula L.), a small bottom-dwelling cyprinid fish, in the littoral zone of Lake Constance, central Europe, was investigated by analysing the mitochondrial control region sequences and five microsatellite loci. An unexpectedly high degree of genetic diversity (up to 0.36%) and old estimated age of these popula...

The aim of the present study was to establish a protocol of sperm cryopreservation in Misgurnus anguillicaudatus and verify the applicability of the obtained protocol in other loach species. We evaluated the following parameters: inseminating dose, thawing temperatures (20, 25 and 30 degrees C for 10s), extenders (loach or cyprinid extenders), internal cryoprotectants (dimethyl sulfoxide (DMSO)...

Current ecological surveys for great crested newts are time-consuming and expensive and can only be carried out within a short survey window. Additional survey methods which would facilitate the detection of rare or protected species such as the great crested newt (Triturus cristatus) would be extremely advantageous. Environmental DNA (eDNA) analysis has been utilized for the detection of great...

Loach minnow (Rhinichthys cobitis) and spikedace (Meda fulgida) are legally protected with the status of Endangered under the U.S. Endangered Species Act and are endemic to the Gila River basin of Arizona and New Mexico. Efficient and sensitive methods for monitoring these species' distributions are critical for prioritizing conservation efforts. We developed quantitative PCR assays for detecti...

circular- crested weirs are employed for flow measurement among their other wide applications in hydraulic engineering. they can also be used to control water level in farm ponds and reservoirs. advantages of the circular crested weirs as compared to other weirs include the stable overflow pattern, the simplicity of design and their associated lower costs. in this study, the curvilinear flow pa...

Measurement of discharge in open channels is one of the main concerns in hydraulic engineering. The structures used for this aim should be accurate, economical and easy to use. Weirs are among the oldest and most convenient hydraulic structures that have been used in both laboratory and field for flow measurement in open channels. Due to limitations of simple sharp crested weirs, recently compo...

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a detailed description of the osteological structure of kiabi loach ( oxynoemacheilus kiabii ), a recently described endemic species caught from gamasiab river, iran, is provided. seven specimens were collected by electrofishing in june 2013 and cleared and stained and its osteological structures were described. the results showed that o. kiabii can be distinguished from other members of the ge...