I was particularly interested in the paper published in Cardiovascular Research (30, 413-418) by Gordon and co-workers entitled: Creatine supplementation in chronic heart failure increases skeletal muscle creatine phosphate and muscle performance. One of the objectives of their study was to evaluate the effects of creatine supplementation (20 g daily for 10 days) on ejection fraction in patient...

Levels of the high-energy phosphate-containing compounds, ATP and creatine phosphate, and of inorganic phosphate (Pi) were measured as a function of position in the cardiac cycle. Measurements were made on isolated, perfused, working rat hearts through the use of gated 31P nuclear magnetic resonance spectroscopy. Levels of ATP and creatine phosphate were found to vary during the cardiac cycle a...

A procedure is described for the determination of the following 19 metabolites in 200 mg gastrocnemius muscle from guinea pig: glucose 1-phosphate and 6-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, 3-phospho- and 2-phosphoglyceric acid, phosphoenolpyruvate, pyruvate, glycerol, glycerol 3-phosphate, AMP, ADP, ATP, inorganic p...

Fedorov P., Grabic R., Fedorova G., Cosson J., Boryshpolets S., Dzyuba B. (2017): Development and application of LC/HRPS for quantification of adenine nucleotides, creatine phosphate, and creatine in sturgeon spermatozoa. Czech J. Anim. Sci., 62, 67–74. The objective of this study was to investigate the applicability of liquid chromatography coupled with high resolution product scan (LC/HRPS) m...

To establish optimum conditions for creatine kinase (EC 2.7.3.2) activity measurement with the creatine phosphate in equilibrium creatine reaction, we re-examined all kinetics factors relevant to an optimal and standardized enzyme assay at 30 and 25 degrees C. We determined the pH optimum in vaious buffers, considering the effect of the type and concentration of the buffer, as well as the influ...

It has been speculated that creatine supplementation affects muscle glucose metabolism in humans by increasing muscle glycogen storage and up-regulating GLUT-4 protein expression. In the present study, we assessed the effects of creatine loading and prolonged supplementation on muscle glycogen storage and GLUT-4 mRNA and protein content in humans. A total of 20 subjects participated in a 6-week...

Glycerinated rabbit psoas fibers were tested for their ability to contract under the influence of creatine phosphate and creatine kinase in the absence of free nucleotide. Tension development by the fibers was observed upon addition of creatine phosphate to the medium containing creatine kinase purified to the first lyophilization stage. However, when the enzyme was washed free of nucleotides b...