At various intervals after plating, cultures were exposed to 2-h pulses of tritium-labeled leucine. For determination of incorporated radioactivity, the creatine kinases were isolated by specific immunoprecipitation. Sodium dodecyl sulfate-gel electrophoresis was used to separate the two types of creatine kinase subunits MCK and B-CK from each other and their radioactivity was determined in the...

Effect of cocaine on leakage of creatine kinase from isolated fast and slow muscles. levels of serum creatine kinase (CK), we determined whether cocaine can . A control group comprised patients who were not cocaine users and satisfied the. RESULTS: The CK level in the cocaine group was 931 +/1,785 U/L (mean . Effects of cocaine on leakage of creatine kinase from skeletal muscle: in vitro. Serum...

The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were...

Strenuous physical activity is able to cost muscle damage. In urban society, ten kilometers running has become a modern culture. It induces acute inflammation as normal physiological response. Conversely, foam roller known self-myofascial release (SMR) technique which can be done individually. Previous studies stated that rolling (FR) could increase flexibility, and reduce pain sensation. Howev...

One hundred patients with chest pain of cardiac origin were evaluated on the basis of clinical findings, electrocardiograph results, and total creatine kinase (CK) and creatine kinase B-subunit (CK-B) activity (as determined by immunoinhibition with the Boehringer CK-MB kit) in serum. All patients diagnosed as having had an acute myocardial infarction had increased values for both CK-B and tota...

Background: Creatine kinase MB (CK-MB), which exists mainly in the cytoplasm of myocardial cells, is currently accepted as an indicator and has high sensitivity specificity for diagnosis injury. Objective: This study was aimed to estimate serum creatine (CK-MB) levels after asphyxia-induced injury neonates. Patients methods: 40 neonates were included this divided into 20 cases with perinatal h...

Creatine kinase (CK; EC 2.7.3.2) isoenzyme BB extracted from brains of rats reportedly undergoes modification at 37 degrees C, leaving an electrophoretic variant that accounts for most of the residual CK activity. This variant, called CK-BB', migrates on electrophoresis similarly to creatine kinase isoenzyme MB. Using electrophoresis and immunoinhibition with antiserum to creatine kinase isoenz...

The effect of gastrointestinal surgery on serum creatine kinase activity was studied in 30 patients. The MB isoenzyme was demonstrated in sera of 30% of the patients and BB isoenzyme in 23%. MB content varied from 0.8 to 10.3% of the total creatine kinase activity, and the BB content from 0.6 to 18.4%. The CK-BB was probably of gastrointestinal origin, since gastrointestinal tract contains high...

the purpose of this investigation was to develop a simple colorimetric method for creatine kinase (ck). the new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and adp, with different glyoxal compounds. hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were s...

The rates of degradation of creatine kinase subunits, M-CK and B-CK subunits, were measured in cultured myogenic cells and in subcultured fibroblasts. In differentiated myogenic cells, the myotubes, both M-CK and B-CK subunits are synthesized. Their rates of degradation were compared. The M-CK subunits is slightly more stable and is degraded with an average apparent half-life of 75 h, whereas t...