This study is designed to isolate and molecular identification of C. neoformans, neoformans pathogenic yeast effect immunocompromised immunocompetent. Methods: collect 50 samples from pigeon dropping fanciers (sputum). The collection time was extended November 2021 February 2022, then culture at SDA, BSA, Cryptococcus Differential agar, esculin Eucalyptus leaves agar media Brain heart infusion ...

A protocol for intact DNA preparation from the basidiomycetous yeast Cryptococcus neoformans has been developed and applied to karyotyping C. neoformans isolates displaying different degrees of capsule formation. A total of 46 strains have been analyzed: 23 (50%) isolated from environmental samples (pigeon droppings), all of them belonging to C. neoformans var. neoformans; and 23 (50%) from cli...

Cryptococcus neoformans is a basidiomycetous, yeast-like fungus which, following inhalation from an environmental source, causes respiratory and neurological disease in humans and animals. Though the ecology of C. neoformans is poorly understood, recent observations provide clues about its life-cycle in nature. Two biotypes of C. neoformans are recognised, C. neoformans var. neoformans (serotyp...

Cryptococcus neoformans is an opportunistic fungal pathogen that primarily causes disease in immunocompromised individuals. Dendritic cells (DCs) can phagocytose C. neoformans, present cryptococcal antigen, and kill C. neoformans. However, early events following C. neoformans phagocytosis by DCs are not well defined. We hypothesized that C. neoformans traffics to the endosome and the lysosome f...

Sixty clinical isolates of Cryptococcus neoformans from AIDS from Goiânia, state of Goiás, Brazil, were characterized according to varieties, serotypes and tested for antifungal susceptibility. To differentiate the two varieties was used L-canavanine-glycine-bromothymol blue medium and to separate the serotypes was used slide agglutination test with Crypto Check Iatron. The Minimal Inhibitory C...

the basidiomycetous yeast genus cryptococcus contains two medically important pathogens, cryptococcus neoformans and c. gattii [1-3]. c. neoformans is one of the common pathogens in acquired immunodeficiency syndrome (aids), whereas the most cases of diseases due to c. gattii happened in the healthy individuals [2, 4]. c. gattii has a tendency to affect the respiratory and nervous systems of th...

Cryptococcus neoformans was first cultivated by Sanfelice from fermenting peach juice in 1894. At the same time, Busse and Buschke reported, separately, the first case of human disease caused by this yeast. During the next years, the fungus was isolated only from lesions or secretions of men or animals. In 1951, Emmons reported the isolation of C. neoformans from soils of Virginia and, in 1955,...

Cell-mediated immunity plays an important role in immunity to the pathogenic fungus Cryptococcus neoformans. However, the antigen specificity of the T-cell response to C. neoformans remains largely unknown. In this study, we used two approaches to determine the antigen specificity of the T-cell response to C. neoformans. We report here that a diverse T-cell receptor (TCR) Vbeta repertoire was m...

Cryptococcus neoformans var. neoformans (74 isolates) and C. neoformans var. gattii (44 isolates) were used to test urease activity after growth on both yeast extract-glucose-peptone agar (YEPG) and on YEPG supplemented with 100 microM EDTA. Every isolate grown on YEPG agar for 48 h at 30 degrees C produced a positive reaction within 1 h in a modified rapid urease assay at 37 degrees C. However...

The aim of this study was to contribute to the knowledge of the geographic distribution of Cryptococcus neoformans varieties, in Colombian patients, and to determine the mating types of such varieties. A total of 370 clinical isolates were studied. C. neoformans var. neoformans was identified in 95.2% of them. C. neoformans var. gattii in 4.8%, 4.5% being serotype B and 0.3%, serotype C. Fifty-...