OBJECTIVE Interleukin 6 (IL6) has the ability to influence each level of the hypothalamo-pituitary-adrenocortical (HPA) axis. The aim of the study was to test whether IL6 concentration correlates with the adrenal cortex response to ACTH in healthy humans. We postulated that higher basal IL6 concentration would be associated with the higher cortisol response to the stimulation. DESIGN AND METH...

Serum cortisol free fraction is responsible for its physiological function. Determination of serum total cortisol concentration does not allow accurate evaluation of hypothalamic pituitary adrenal axis when there is a quantitative variation in serum transcortin. Although free cortisol can be assayed by salivary or urinary cortisol measurements, these methods can not be easily applied in intensi...

The mechanisms of action of o,p'-DDD on adrenal steroidogenesis were investigated in vitro in rainbow trout (Oncorhynchus mykiss). Acute exposures to o,p'-DDD inhibited ACTH-stimulated cortisol secretion while cell viability decreased significantly only at the highest concentration tested (200 microM o,p'-DDD). Stimulation of cortisol secretion with a cAMP analogue (dibutyryl-cAMP) was inhibite...

Cortisol plays an important part in physiological procedures. Observing cortisol concentration as a stress indicator demonstrates the mode for diagnosis goal. The mechanism of presented nano biosensor is LSPR and performed based on nucleic acid aptamer functionalized with gold nanoparticles which have specific interaction cortisol. particular measurement was accomplished over from 0.05 -0.2 μg·...

OBJECTIVE Patients with glucocorticoid deficiency need lifelong glucocorticoid replacement treatment. During acute stressful events, steroid dosage must be increased several times, which is often problematical in children. This study investigated the reliability of rectal hydrocortisone administration as an alternative to the intramuscular route. STUDY DESIGN Serum cortisol was assessed durin...

introduction the dexamethasone test has been widely used for diagnosing hypercortisolism. materials and methods we assessed the relationship between the basal and suppressed cortisol values in urine and plasma during a low-dose dexamethasone test in patients with proved cushing&apos;s disease. results a statistically highly significant correlation was found (for urine cortisol: r = 0.66, p <0.0...

This paper presents a comprehensive investigation of the various parameters involved in fabrication molecularly imprinted polymer (MIP) sensor for detection cortisol. Parameters such as monomer concentration, electropolymerization cycles, pH, monomer–template ratio, template removal technique, and rebinding time were optimized to establish more consistent effective method MIP sensors. Under con...