The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructose-egg yolk diluent to increase Awassi ram sperm storage period at 5 ˚C. Semen samples from six mature Awassi rams were used in this study. The semen samples were diluted by Tris-glucose-egg yolk. Diluted semen sample was divided into three parts. The first part was added with 0.9 mg mL(-1) vitamin C, the s...

The present study was undertaken to investigate the effect of various levels of vitamin B12 in Tris extender on semen quality of Dallagh rams following the cooling and freeze/thawing process. Semen was collected from six healthy and mature rams with an average body weight of 60.0 ± 5.0 Kg using an electro ejaculator. High quality samples were mixed and diluted in Tris extender supplemented with...

Seminal plasma transaminases (GOT and GPT) were assessed in diluted (Tris-egg yolk-citric acid-fructoseglycerol extender 1:4) and thawed (40 0 C for 15 seconds) semen of Sirohi bucks (control group). Diluted, cooled (from 25 0 C up to 5 0 C) and equilibrated (at 5 0 C for 2 hours) semen straws were frozen @ 15, 20, 25 and 30 0 C/minute for M1, M2, M3 and M4 groups respectively. These frozen str...

Artificial insemination (AI) using fresh diluted semen is used worldwide in the porcine industry. Viability of spermatozoa in diluted semen depends on several factors, such as the interaction with the type of extender and storage duration. The aim of this study was to evaluate the effects of extenders and storage time on the motility and ultrastructure of cooled-preserved boar spermatozoa. Seme...

Semen from the first 15mL of the ejaculate (P1) obtained from two boars (30mL) was diluted in glycineegg yolk extender, cooled at 5°C in a special container and rediluted in standard doses of 3x10 mobile spermatozoa after 12h of storage. Semen was also stored up to 24h after redilution. The physical characteristics of the semen were evaluated at different storage periods (fresh, 0h, 12h, redilu...

This review summarises the most significant findings related to the issue of the quantitative and qualitative indicators of stallion ejaculates, its processing when producing chilled insemination doses and the possibility of influencing the individual steps of the entire process. Artificial insemination, with cooled-storage semen, is increasingly used in horse breeding because it offers many ad...

Semen, collected from boars of proven fertility, was concentrated by centrifuging the strained ejaculate at 400 g for 15 min at 20\s=deg\C and gently resuspending the sperm pellet in one-fifth the original volume of seminal plasma. The concentrated semen was then diluted 1:6 either in a chosen wash medium (see Table 1 for composition of media) and used immediately, or in a milk diluent (MFP), c...

It is now possible to store turkey semen for 4 to 24 h without appreciable loss in fertility and hatchability. Optimum conditions for 24 or 48 h storage have not been fully determined. Development of new storage media, allowing the insemination of very low numbers of spermatozoa, may provide interesting possibilities regarding the use of superior sires. This study was conducted to evaluate effe...