نتایج جستجو برای: conventional culture method

تعداد نتایج: 2069477  

Journal: :iranian journal of veterinary research 2015
p. r. shivahre a. k. gupta a. panmei b. r. yadav m. bhakat

in vitro fertility assessment using fluorescent technique is a better predictor of fertility status of bulls as compared to traditional semen quality assessment techniques, therefore, the study was planned to assess in vitro fertility status of bulls based on conventional and fluorescent techniques. seventy-three ejaculates were collected from 12 murrah buffalo bulls maintained at artificial br...

Journal: :iranian journal of veterinary medicine 2011
عباسعلی ساری abdollah hamshidi mohammadreza basami

campylobacter jejuni is a major cause of food-borne diarrhea in many countries. poultry and poultry products are known as important sources of human campylobacteriosis. in this study, conventional culture and multiplex pcr methods were compared for the detection of c. jejuni isolated from poultry carcasses. a total of 100 samples, representing 20 broiler flocks, were collected from poultry carc...

Journal: :Journal of clinical microbiology 1989
M Bobadilla J Sifuentes G Garcia-Tsao

The definitive diagnosis of spontaneous bacterial peritonitis is made by a positive ascitic fluid culture. Causative organisms cannot be isolated in up to 65% of patients with well-defined spontaneous bacterial peritonitis, probably due to inadequate ascites culture techniques. We prospectively compared two ascites culture methods: conventional (on chocolate agar and thioglycolate broth) and mo...

Journal: :Journal of clinical pathology 1999
A J Simpson P A Howe V Wuthiekanun N J White

AIMS To determine whether quantitative blood culture methods could improve the diagnosis of septicaemic melioidosis. METHODS A comparison of conventional broth based blood cultures, a pour plate method, and a commercial lysis centrifugation (Isolator 10) blood culture system was conducted in 71 Thai patients with severe melioidosis. The time to identification of B pseudomallei was recorded fo...

2010
Kenia Barrantes Jiménez Clyde B. McCoy² Rosario Achí

A Multiplex Polymerase Chain Reaction (PCR) assay to be used as an alternative to the conventional culture method in detecting Shigella and enteroinvasive Escherichia coli (EIEC) virulence genes ipaH and ial in lettuce was developed. Efficacy and rapidity of the molecular method were determined as compared to the conventional culture. Lettuce samples were inoculated with different Shigella flex...

2014
Valeria Velasco Julie S. Sherwood Pedro P. Rojas-García Catherine M. Logue

The aim of this study was to compare a real-time PCR assay, with a conventional culture/PCR method, to detect S. aureus, mecA and Panton-Valentine Leukocidin (PVL) genes in animals and retail meat, using a two-step selective enrichment protocol. A total of 234 samples were examined (77 animal nasal swabs, 112 retail raw meat, and 45 deli meat). The multiplex real-time PCR targeted the genes: nu...

Journal: :iranian journal of veterinary science and technology 0

various techniques and culture media were developed for rapid identification of salmonella serovars. however, there are still problems with their sensitivity and specificity. in an attempt to reduce the time spent to obtain a result and to minimize the problems associated with rapid detection systems such as interference from food ingredients debris, micro flora in feces, and lack of sensitivit...

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