Cortical compartments partition proteins and membrane at the cell surface to define regions of specialized function. Here we ask how cortical compartments arise along the plasma membrane furrows that cellularize the early Drosophila embryo, and investigate the influence that this compartmentalization has on furrow ingression. We find that the zygotic gene product Nullo aids the establishment of...

The physical interaction of the plasma membrane with the associated cortical cytoskeleton is important in many morphogenetic processes during development. At the end of the syncytial blastoderm of Drosophila the plasma membrane begins to fold in and forms the furrow canals in a regular hexagonal pattern. Every furrow canal leads the invagination of membrane between adjacent nuclei. Concomitantl...

Plasma membrane furrow formation is crucial in cell division and cytokinesis. Furrow formation in early syncytialDrosophila embryos is exceptionally rapid, with furrows forming in as little as 3.75 min. Here, we use 4D imaging to identify furrow formation, stabilization, and regression periods, and identify a rapid, membrane-dependent pathway that is essential for plasma membrane furrow formati...

In cleaving eggs of the newt, Cynops pyrrhogaster, the behaviour of the egg surface was analysed by observing linear and area changes in a transplanted piece of cortex which differed in pigmentation from the background. Results from the animal hemisphere showed that the surface in the cleavage plane shrank extensively in the region where the early cleavage furrow was being formed. On both sides...

Despite extensive work on the mechanisms that generate plasma membrane furrows, understanding how cells are able to dynamically regulate furrow dimensions is an unresolved question. Here, we present an in-depth characterization of furrow behaviors and their regulation in vivo during early Drosophila morphogenesis. We show that the deepening in furrow dimensions with successive nuclear cycles is...

Females homozygous for a mutation in cellular island (cei) produce embryos with defects in cytokinesis during early development. Analysis of the cytoskeletal events associated with furrow formation reveal that these defects include a general delay in furrow initiation as well as a complete failure to form furrow-associated structures in distal regions of the blastodisc. A linkage mapping-based ...

The overgrazing and excessive utilization of rangelands has caused vegetationand soil degradation in many rangelands. In the rangeland management, the soil andvegetation elements, environmental factors and livestock should be considered as decisionmaking agents. Various methods are applicable for the rangeland restoration as contourfurrows and mortar stone dam. In order to evaluate the effects ...

Response to the insect hormone ecdysone is mediated by a nuclear receptor complex containing Ultraspiracle (USP) and the Ecdysone Receptor (EcR). Among other phenotypes, loss of functional USP in Drosophila eye development results in an accelerated morphogenetic furrow, although loss of ecdysone arrests the furrow. We have shown that USP both represses and activates a gene affecting furrow move...

Furrow irrigation systems have a greater application capacity, are less costly, and use less energy than sprinkler systems but furrow irrigation produces greater runoff, erosion, and deep percolation losses. Phosphorus (P) and nitrogen (N) losses are associated with runoff sediment, and can be minimized by eliminating irrigationinduced erosion. Excessive leaching of inorganic and organic solute...

The mitotic spindle determines the cleavage furrow site during metazoan cell division, but whether other mechanisms exist remains unknown. Here we identify a spindle-independent mechanism for cleavage furrow positioning in Drosophila neuroblasts. We show that early and late furrow proteins (Pavarotti, Anillin, and Myosin) are localized to the neuroblast basal cortex at anaphase onset by a Pins ...