The genus Ranunculus L., with a worldwide distribution, is the largest member of the Ranunculaceae. Here, nuclear ribosomal internal transcribed spacer (ITS) sequence data and chloroplast trnLF sequence data were used to analyze phylogenetic relationships among members of the annual and perennial (Group Praemorsa, Group Rhizomatosa, Group Grumosa and Group non-Grumosa) species of Ranunculus...

Karyopherinb (Kapb) proteins bind nuclear localization and export signals (NLSs and NESs) to mediate nucleocytoplasmic trafficking, a process regulated by Ran GTPase through its nucleotide cycle. Diversity and complexity of signals recognized by Kapbs have prevented prediction of new Kapb substrates. The structure of Kapb2 (also known as Transportin) bound to one of its substrates, the NLS of h...

the genus ranunculus l., with a worldwide distribution, is the largest member of the ranunculaceae. here, nuclear ribosomal internal transcribed spacer (its) sequence data and chloroplast trnlf sequence data were used to analyze phylogenetic relationships among members of the annual and perennial (group praemorsa, group rhizomatosa, group grumosa and group non-grumosa) species of ranunculus...

The problem of multiple global comparison in families of biological sequences has been wellstudied. Fewer algorithms have been developed for identifying local consensus patterns or motifs in biological sequence. These two important problems have di erent biological constraints and, consequently, di erent computational approaches. The di culty of nding the biologically meaningful motifs results ...

Here we report the analysis of dual G-quadruplexes formed in the four repeats of the consensus sequence from the insulin-linked polymorphic region (ACAGGGGTGTGGGG; ILPR(n)(=4)). Mobilities of ILPR(n)(=4) in nondenaturing gel and circular dichroism (CD) studies confirmed the formation of two intramolecular G-quadruplexes in the sequence. Both CD and single molecule studies using optical tweezers...

The consensus sequence of the third histidine box of a range of A,, A6, An and sphingolipid desaturases differs from that of the membrane-bound non-fusion A'' and A15 desaturases in the presence of glutamine instead of histidine. We have used site-directed mutagenesis to determine the importance of glutamine and other residues of the third histidine box and created a chimaeric enzyme to determi...

4 Visualising oligonucleotide and consensus sequence densities 4 4.1 Preparing input sequences . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 4.2 Plotting dinucleotide density maps . . . . . . . . . . . . . . . . . . . . . . 6 4.3 Plotting average oligonucleotide profiles . . . . . . . . . . . . . . . . . . . . 10 4.4 Plotting consensus sequence density map . . . . . . . . . . . . . . ...

In investigating sequence variants in a family of highly repeated rat DNA, we needed to search the consensus sequence of the repeat unit of this family for short sequences which would become, with one base change, recognition sites for various restriction endonucleases. To do this, we have designed a pair of programs to search DNA sequences of any length for sets of related short sequences, all...