Confocal microscopy, which is a major advance upon normal light microscopy, has been used in a number of scientific fields. By confocal microscopy techniques, cells and tissues can be visualized deeply, and three-dimensional images created. Compared with conventional microscopes, confocal microscope improves the resolution of images by eliminating out-of-focus light. Moreover, confocal microsco...

A new type of confocal fluorescence microscope with an unprecedented resolution is proposed. Calculations demonstrate that an axial resolution on the order of 100 nm can be achieved in a confocal microscope with enlarged aperture when two-photon excitation is applied. This axial resolution is up to four times higher than that ofa confocal fluorescence microscope. The proposed microscope yields ...

A straightforward and transparent model, based on Gaussian beam optics, for the axial r0 resolution of a confocal microscope is presented. A confocal Raman microscope was used to determine the axial confocality in practice. The axial response of a thin planar object was measured for three different objectives, two pinhole sizes and a slit. The results show that, in the case of a confocal config...

background: the aim of this study was to evaluate and compare the in vitro biofi lm forming capacity of enterococcus faecalis on gutta-percha points disinfected with four disinfectants. materials and methods: a total of 50 gutta-percha points used in this study were divided into four test groups based on disinfectant (5.25% sodium hypochlorite, 2% chlorhexidine gluconate, 20% neem, 13% benzalko...

A diffractive zone plate provides a highly linear wavelength-to-depth coding, allowing for nonmechanical depth scanning in a confocal microscope. This chromatic confocal microscope, constructed with 40x and 60x objectives, achieves axial position changes of 55 and 25 mum, respectively, for a wavelength tuning range of 100 nm. The corresponding longitudinal point-spread functions are measured an...

The Scanning Confocal Electron Microscope, is an instrument which permits the observation and characterization of sub-surface structures of thick, optically opaque materials at nanometer level resolutions. The instrument merges the capabilities of the scanning, transmission and x-ray microscopes, and achieves unprecedented resolutions in optically dense materials, by implementing the technology...

We describe the three-dimensional (3-D) image formation and data acquisition in a stage scanning 4Pi confocal fluorescence microscope with the use of two-photon excitation. The 3-D point-spread functions of the 4Pi confocal and regular confocal microscope are measured and compared. Particular emphasis is given to the data acquisition procedure. 4Pi confocal microscopy results in a point-spread ...