Although much progess has been made in the detection and characterization of homocytotropic antibodies, identification of the factors which control their synthesis remains to be determined. To assess the influence of different adjuvants on anti-dinitrophenol (DNP) immunoglobulin E (IgE) antibody responses, rabbits were immunized with adjuvant plus homologous albumin (HRA) heavily substituted wi...

For many decades, mineral oils with or without dried mycobacteria, i.e. Complete and Incomplete Freund ́s Adjuvant, have been the most commonly used immune adjuvants for experimental work. Later on, better defined hydrocarbon oils such as pristane (2,6,10,14-tetramethylpentadecane), squalene (2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosa-hexane), or hexadecane have emerged and partially r...

  Partially fed (4 -5 days) H .a. anatolicum (Iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (GS), gut membrane antigen (GM), triton extracted gut membrane antigen ( TX-GM ) and sodium dodecyl sulphate treated Triton extracted gut membrane antigen (SDS-TX-GM). The New Zealand white rabbits were immunized with GS, GM, TX- ...

Although adjuvants are important components of vaccines, few studies have been conducted to establish the criteria on adjuvant selection and to investigate mechanisms of adjuvant actions during vaccination. Here we found that complete Freund adjuvant (CFA) induced a CD11b cell population in a B-cell independent manner. This cell population exhibited strong ability to inhibit T-cell-mediated rej...

partially fed (4 -5 days) h .a. anatolicum (iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (gs), gut membrane antigen (gm), triton extracted gut membrane antigen ( tx-gm ) and sodium dodecyl sulphate treated triton extracted gut membrane antigen (sds-tx-gm). the new zealand white rabbits were immunized with gs, gm, tx- gm...

Crotalaria juncea (Leguminoceae) has been used for its antiarthritic effect in the folklore medicine. The purpose of this study was investigating experimentally the possible antiarthritic and antiulcerogenic properties of Crotalaria juncea leaves in animal model. Ethanolic extract of Crotalaria juncea (CJE) was administered at the doses of 200 and 400 mg/kg to complete Freund’s adjuvant (...

partially purified antigenic fractions of leishmania major promastigotes were obtained by sodium dodecyl sulfate (sds-page) and electro-elution. three isolated protein fractions designated as frac tions (fr.) 1, 2, and 3 correspond to 40-60, 60-80, 80-100 kilodalton (kda) respectively. they were used for immunization of balb/c mice against leishmaniasis. the effects of these fractions on immune...

In order to replace the use of mammals for the production of hyperimmune sera and to replace Freund"s complete adjuvant (FCA) with its undesirable side effects, the production and immunological features of IgY were investigated systematically. An important advantage of IgY is that large amounts can easily be extracted from the yolk of immunized laying hens. With special regard to animal welfare...