This protocol describes the loading of individual cells with fluorescent probes via patch pipettes. The patch-clamp methodology has been successfully used for single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations. A broad range of dyes can be used with this loading technique. Markers for morphological reconstruction (e.g., Lucifer yellow); ion-sensitive indicator ...

here we report a novel approach for preparation of a 6-day transdermal drug delivery system (tdds) as treatment for mild to moderate alzheimer’s disease. the spray drying method was used to prepare microparticles containing the anti-alzheimer drug. the content of the drug was determined by high performance liquid chromatography (hplc) method. the morphology and size range of the microparticles ...

in this paper, simulation and analysis of thin steel cylindrical shells with elliptical cutouts under oblique loading were studied using finite element method. first, the numerical results were validated by the results of experimental test performed by an instron 8802 servo hydraulic machine. also, the effect of cutout angle (θ), cutout size, cutout position (l0/l) and cutout aspect ratios (b/a...

Simple, accurate buckling interaction formulae are presented for long orthotropic plates with either simply supported or clamped longitudinal edges and under combined loading that are suitable for design studies. The loads include 1) combined uniaxial compression (or tension) and shear, 2) combined pure inplane bending and 3) shear and combined uniaxial compression (or tension) and pure inplane...

are compared with the experimental results. In these test results the shape memory alloys behavior as: super elasticity under various temperatures, loading rate effects, asymmetry in tension and pressure, various loops of loading and unloading, hydrostatic pressure effects, different proportional tension-shear biaxial loading and unloading, and also deviation from normality due to non-proportio...

Gecko-inspired microfibre arrays with 42 million polypropylene fibres cm(-2) (each fibre with elastic modulus 1 GPa, length 20 microm and diameter 0.6 microm) were fabricated and tested under pure shear loading conditions, after removing a preload of less than 0.1 N cm(-2). After sliding to engage fibres, 2 cm2 patches developed up to 4N of shear force with an estimated contact region of 0.44 c...

Here we report a novel approach for preparation of a 6-day transdermal drug delivery system (TDDS) as treatment for mild to moderate Alzheimer’s disease. The spray drying method was used to prepare microparticles containing the anti-Alzheimer drug. The content of the drug was determined by High Performance Liquid Chromatography (HPLC) method. The morphology and size range of the microparticles ...

in this paper, the effect of cyclic loading on punching strength of flat slabs strengthened with carbon fiber reinforced polymer (cfrp) sheets is studied. experimental results of ten slab specimens under monotonic and cyclic loading are analyzed. eight specimens were strengthened with cfrp sheets on the tensile face of the slabs and the two other specimens were kept un-strengthened as control s...

carbonate materials are mostly found in tropical areas, where exploiting gas and oil resources are of high concern. their unique behavior under shear loading first was recognised during oil resources investigations in the persian gulf. off-shore structures have been placed on carbonate soils which are highly crushable.during storms cyclic loading imposes on the bases of structures lied down on ...