We investigated the in vitro effects of ASTA-Z-7595, ASTA-Z-7557, ASTA-Z-7654, and 4-hydroperoxycyclophosphamide (4HC) on murine stromal fibroblastoid colony-forming units, committed hemopoietic progenitors (erythroid burst-forming units and granulocyte/macrophage colony-forming units), and pluripotent hemopoietic stem cells assayed by the spleen colony-forming unit (CFU-s) assay. In general, t...

Erythroid progenitors from 1 8 fetal livers (7-1 5-wk gestational period) and 1 3 normal adult marrows were grown in methylcellubose cultures containing erythropoietin (Ep) and burst-enhancing factor(s) (BEF). Additional experiments were carried out on 1 fetal, 1 5 neonatal. and 26 adult blood specimens. Three classes of progenitors (primitive or mature erythroid burst-forming unit(s). P-BFU-E....

To compare the clonogenicity and distribution of CD34+ subsets in bone marrow (BM), granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood (PB) and cord blood (CB), we analyzed in vitro colony formation and CD34+ cells co-expressing differentiation molecules (CD38, HLA-DR), myeloid associated molecules (CD13, CD33), a T-cell associated molecule (CD3), and a B-cell associated m...

the aim of present study was to investigate the effect of individual and co-inoculation of trichoderma species and arbuscular mycorrhizal fungi (amf) on growth, mycorrhization, population of trichoderma, and wilt disease severity in pigeon pea (cajanus cajan l millsp). three species of trichoderma, namely, t. harzianum (th), t. virens (tr), and t. viride (tv) and consortium of amf (myc; mixture...

Injection of testosterone, nortestosterone, or oxymetholone markedly enhanced granulopoietic recovery in mice made neutropenic by l,3-bis(2-chloroethyl)-l-nitrosourea, vinblastine, or cyclophosphamide. Blood neutrophils rose to six times normal counts, and the bone marrow showed sequential increases first in dividing granulocytic elements and later in the nondividing reserve. The steroid-induce...

Injection of testosterone, nortestosterone, or oxymetholone markedly enhanced granulopoietic recovery in mice made neutropenic by l,3-bis(2-chloroethyl)-l-nitrosourea, vinblastine, or cyclophosphamide. Blood neutrophils rose to six times normal counts, and the bone marrow showed sequential increases first in dividing granulocytic elements and later in the nondividing reserve. The steroid-induce...

Human multilineage colony-stimulating factor (multi-CSF)/interleukin-3 (IL-3) induces colony formation from CFU-GEMM, BFU-E, and CFU-Eo when applied to in vitro cultures of highly enriched hematopoietic progenitor cells. No granulocytic colonies are formed in response to IL-3. However, with appropriate assays, we demonstrate that IL-3 increases the size of G-CSF-induced granulocytic colonies; t...

Erythroid progenitor cells, CFU-E (colony-forming-unit-erythroid), were isolated to practical homogeneity by a combination of three enrichment procedures. CFU-E were generated in large amounts in spleens of mice previously bled and treated with the erythropoiesis-suppressing drug thiamphenicol. The average CFU-E concentration in spleens from mice 4 d after the thiamphenicol-treatment was 10%. T...

The fungal population and distribution of aflatoxin-producing fungi in 30 samples of imported almond powder products purchased from retail markets were examined in this study. Total counts of fungi ranged from under 1.0 x 10 colony-forming units (CFU)/g to 8.5 x 10(3) CFU/g as determined with the dilution plating technique. The predominant fungi in the mould-contaminated almond samples were Asp...

On addition of DMSO, the MEL cell line TSA8 becomes committed into erythroid progenitor cells (CFU-E) which can form differentiated colonies in the presence of erythropoietin. To understand the mechanism of cellular commitment, the number and the affinity of the receptors for erythropoietin were estimated. The affinity of the receptors did not change before or after induction. The number of rec...