The influence of antiplatelet glycoprotein (GP) antibodies on megakaryocytopoiesis in patients with idiopathic or immune thrombocytopenic purpura (ITP) has been well studied. However, the influence of GP antibodies on proplatelet formation is poorly understood. Here we investigated whether in vitro human megakaryocyte colony formation and proplatelet formation are affected by various monoclonal...

The influence of antiplatelet glycoprotein (GP) antibodies on megakaryocytopoiesis in patients with idiopathic or immune thrombocytopenic purpura (ITP) has been well studied. However, the influence of GP antibodies on proplatelet formation is poorly understood. Here we investigated whether in vitro human megakaryocyte colony formation and proplatelet formation are affected by various monoclonal...

Background: Radiotherapy is an established therapeutic modality for prostate cancer. Resveratrol, a natural antioxidant, has been shown to inhibit carcinogenesis and to block the process of tumor initiation and progression. No data is available on the response of cellular spheroid to Reseveratol. In this study we have examined the effect of Resveratol on the radiation response of human p...

a multiple colorimetric assay has been introduced to evaluate the proliferation and determination of survival fraction (sf) of irradiated cells. the estimation of sf based on the cell‑growth curve information is the major advantage of this assay. in this study, the utility of multiple‑mts assay for the sf estimation of irradiated ht‑29 colon cancer cells, which were plated before irradiation, w...

background: radiotherapy is an established therapeutic modality for prostate cancer. resveratrol, a natural antioxidant, has been shown to inhibit carcinogenesis and to block the process of tumor initiation and progression. no data is available on the response of cellular spheroid to reseveratol. in this study we have examined the effect of resveratol on the radiation response of human prostate...

Spleen colony formation after transplantation of bone marrow cells into irradiated mice has been used as an assay for hematopoietic stem cells (CFU-S), but has serious limitations intrinsic to an in vivo assay. In this report we describe experiments using an in vitro clonogenic assay that is especially suitable for studies of stem cell regulation as defined growth factors and normal untreated b...

conclusions flushing dental unit waterlines are an effective measure in reducing the number of bacterial contaminants. results bacterial contamination was found in all 24 units at the beginning of daily activity with a mean colony count of 17850. the number of colonies decreased to 3250 following 30 seconds of flushing in 16 units and later to 1837 after flushing for two minutes in 13 units. in...

background: the present study was designed to evaluate the homing potential of mouse embryonic stem cells (esc) treated with erythropoietin (epo) in hematopoietic organs such as spleen and liver after transplantation using morphological and immuno-histochemical techniques. methods: day-four embryoid body (eb)-derived cells were dissociated and re-plated in medium in the presence and absence of ...

Wilms tumor (WT) is the most common pediatric malignant primary renal tumor. Carboplatin (CRB), a platinum compound is widely used in the treatment of multiple cancers including ovarian, lung, head and neck, and wilm’s tumor. However lower uptake of CRB in cancer cells and toxicity concerns in healthy cells often limited its clinical outcome. The aim of this study was to investigate the antitum...