نتایج جستجو برای: colony assay
تعداد نتایج: 273197 فیلتر نتایج به سال:
the objective of this study was to evaluate the effect of the sto feeder layer on prepubertal rhode island red rooster sscs culture and proliferation in vitro. testis cells from 30 prepubertal rhode island red chicken (4-8 weeks of age), were individually separated and cultivated in the presence of bfgf and lif growth factors on four well plates with two treatments and three replicats and five...
Purpose: Differentiation of Mouse embryonicstem cells into Hematopoietic cells. Materials and Methods: In this study, we used EB formation system for Hematopoietic differentiation of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed ...
Soft agarose culture human tumour colony forming assay for drug sensitivity testing: [3H]-Thymidine incorporation vs colony counting Summary In vitro drug sensitivity testing, both by optical colony counting and by a [3H]-TdR incorporation assay, was performed on human tumour cells proliferating in soft agar cultures. Cells from two different human tumour cell lines, 5 different human tumour xe...
Methodological development of a clonogenic assay to determine endothelial progenitor cell potential.
The precise and conceptual insight of circulating endothelial progenitor cell (EPC) kinetics is hampered by the absence of an assay system capable of evaluating the EPC differentiation cascade. An assay system for EPC colony formation was developed to delineate circulating EPC differentiation. EPC colony-forming assay using semisolid medium and single or bulk CD133(+) cells from umbilical cord ...
Spleen colony formation after transplantation of bone marrow cells into irradiated mice has been used as an assay for hematopoietic stem cells (CFU-S), but has serious limitations intrinsic to an in vivo assay. In this report we describe experiments using an in vitro clonogenic assay that is especially suitable for studies of stem cell regulation as defined growth factors and normal untreated b...
Objective(s):In previous investigations, we have shown para-nonylphenol (p-NP) caused significant reduction of proliferation and differentiation of rat bone marrow mesenchymal stem cells (MSCs) in vitro. In this study, we first treat the rats with p-NP, then carried out the biochemical and morphological studies on MSCs. Materials and Methods: Proliferation property of cells was evaluated with t...
objective(s):in previous investigations, we have shown para-nonylphenol (p-np) caused significant reduction of proliferation and differentiation of rat bone marrow mesenchymal stem cells (mscs) in vitro. in this study, we first treat the rats with p-np, then carried out the biochemical and morphological studies on mscs. materials and methods: proliferation property of cells was evaluated with t...
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