In order to study of kidney tubules, twenty specimen of 45 days old juvenile grouper (Epinephelus coioides) were prepared after proper biometry. Five mm thick sections were removed from the middle and posterior end of the kidney and fixed in Bouin and glutaraldehyde. The routine procedures of preparation of tissues were followed for light and transmission electron microscopic study. In macrosco...

Addisonian patients can maintain potassium homeostasis despite the absence of mineralocorticoid. The present in vitro microperfusion studies examine what role the cortical collecting tubule might play in this process. All studies were performed on tubules harvested from adrenalectomized rabbits, which were maintained on 0.15 M NaCl drinking water and dexamethasone 50 mug/d. Perfusion and bath s...

HELMAN, S. I., J. J. GRANTHAM, AND M. B. BURG. Eject of vasopressin on electrical resistance of renal cortical collecting tubules. Am. J. Physiol. 220(6) : 1825-1832. 197 1 .-A method is reported for measuring the transepithelial potential difference and electrical resistance in isolated rabbit renal cortical collecting tubules. The mean resistance with isotonic perfusion fluid was 13.8 X lo4 o...

Miller, R. Lance, Ping Zhang, Tong Chen, Andreas Rohrwasser, and Raoul D. Nelson. Automated method for the isolation of collecting ducts. Am J Physiol Renal Physiol 291: F236–F245, 2006. First published February 7, 2006; doi:10.1152/ajprenal.00273.2005.—The structural and functional heterogeneity of the collecting duct present a tremendous experimental challenge requiring manual microdissection...

Congenital polycystic kidney disease is characterized by the formation of large fluid-filled cysts in kidney tubules. It has been postulated that increased epithelial cell proliferation and altered transtubular fluid transport are necessary for cyst formation. To address the latter problem, we have studied the plasma membrane distribution of the alpha 1 and beta 1 subunits of Na+/K(+)-ATPase du...

The structural and functional heterogeneity of the collecting duct present a tremendous experimental challenge requiring manual microdissection, which is time-consuming, labor intensive, and not amenable to high throughput. To overcome these limitations, we developed a novel approach combining the use of transgenic mice expressing green fluorescent protein (GFP) in the collecting duct with larg...

BURG, MI., L. STONER, J. CARDINAL, AND N. GREEN. Furosemide effect on isolated perfused tubules. Am. J. Physiol. 225(l): 119-124. 1973 .-Proximal convoluted tubules, thick ascending limbs of Henle’s loop, and cortical collecting tubules were dissected from rabbit kidneys, and perfused in vitro. The effect of the diuretic agent furosemide on electrolyte transport and electrical potential differe...

a certain point of the inner medulla, and H(ƒÔ) and A(ƒÔ) give the total internal circumferences in mm of Henle's thin segments and collecting tubules respectively on a curved surface defined by ƒÔ. As indicated by different exponents in H(ƒÔ) and A(ƒÔ), quantitative predominance of Henle's thin segments over collecting tubules toward the external border of the inner medulla was demonstrated. T...